Cationic graft-copolymer for non-viral gene delivery vector

A non-viral gene delivery vector formed from an aqueous solution of a cationic graft-copolymer of a water-soluble linear backbone polymer having hydroxyl groups, for a non-viral gene delivery vector, comprising a unit derived from a cationic water-soluble linear polysaccharide of the following formula (1)

• [C6H7O2(OH)3-a(OX)a]xH2O (1)
• or a unit derived from a water-soluble linear polyvinylalcohol of the following formula (2) or a partial hydrolyzed alcohol of the following formula (3)
• —[CH2CH(OH)1-b(OX)b]n— (2)
• —[CH2CH(OH)1-b-c(OX)b(OAc)c]n— (3),
• wherein X is a —(CH2)mR1 organic radical where R1 is a member of the class consisting of:
• —NH2 radical,
• —N(CH3)2 radical,
• —N(C2H5)2 radical,
• —N+(C2H5)3 radical,
• —N+(CH2)2CH2CH(OH)CH3 radical,
• —N+(C2H5)2CH2CH(OH)CH3 radical,
• —N+(C2H5)2(C2H5)N(C2H5)2 radical,
• —C6H4NH2 radical,
• —COC6H4NH2 radical,
• —COR2 radical where R2 is —CH2NH2 or —C6H4NH2, and
• —CH2CH(OH)CH2R3 radical where R3 is —NH2, —N(CH3)2, —N(C2H5)2, or —N+(C2H5)3 radical,
• where m is a natural number of 1 to 3, a is a positive number having a value of 0<a<3, b is a positive number having a value of 0<b<1, x and n are natural numbers having a value of 5 or more, 1>b+c, and Ac is acetyl radical;
• and
• a unit derived from a polymerize-able olefin compound of the following formula (4):
• wherein R4, R5 and R6 are each selected from the group consisting of hydrogen and CH3, and R7 is a member of the group consisting of:
• where R8 is a member of the class consisting of:
• hydrogen, C1-C12 alkyl radicals, cyclohexyl radical, C1-C4 hydroxyalkyl radicals, C1-C8 aminoalkyl radicals, C1-C8 dialkylaminoalkyl radicals, glycidyl radical, tetrahydrofuran radical, C1-C4 lower alkyl-substituted tetrahydrofuran radical, benzyl radical, a (CH2CH2O)yCH2CH2OH radical where y is a positive integer from 1 to 10, and —N(R9)2, where the two R9's which may be the same or different, are either hydrogen or a C1-C4 alkyl radical;
• wherein R10 is a C1-C8 alkyl radical, phenyl radical, tolyl radical, pyridine radical, pyrrolidone radical;
• and
• where R11 is NH2, NHCH3, N,N-dimethylamino radical, N,N-dimethylaminopropylamino radical, and morpholine radical.