High-level expression of recombinant antibody in a mammalian host cell
OwnerJames Rance, Stephan Kalwy +2inventors
InventorsJames Rance, Stephan Kalwy, Robert Young, Robert Gay
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Abstract
A mammalian host cell with a high-level expression of recombinant antibody containing a double-gene vector with gene optimized nucleotide sequences encoding the light and the heavy antibody chains. The double-gene vector itself. A process for enhancing the production of recombinant antibody in a mammalian host cell.
Claims49
A mammalian expression vector comprising at least a first transcription unit containing a first synthetic nucleotide sequence encoding a first polypeptide chain and a second transcription unit containing a second synthetic nucleotide sequence encoding a second polypeptide chain wherein the first and second synthetic nucleotide sequences are based on naturally occurring nucleotide sequences and wherein the first and second polypeptide chain can form a molecule comprising at least one or multiple copies of each of the first and the second polypeptide chain, the codon composition of both the first and second nucleotide sequence is adapted to the codon bias of the genes of a given mammalian host species which is different from the mammalian species from which the naturally occurring nucleotide sequences originally were derived.
A mammalian expression vector comprising at least a first transcription unit containing a first synthetic nucleotide sequence encoding a first polypeptide chain and a second transcription unit containing a second synthetic nucleotide sequence encoding a second polypeptide chain wherein the first and second synthetic nucleotide sequences are based on naturally occurring nucleotide sequences and wherein the first and second polypeptide chain can form a molecule comprising at least one or multiple copies of each of the first and the second polypeptide chain, the codon composition of both the first and second nucleotide sequences is amended such that their mRNAs are translated with higher efficiency.
A mammalian expression vector according to claim 25, wherein the two synthetic nucleotide sequences contain less internal TATA-boxes, chi-sites and/or ribosomal entry sites.
Dependencies are inferred from the claim text.