# US 2009/0215162 A1 · Nucleic acid amplification device

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- Number: US 2009/0215162 A1
- Document ID: US20090215162A1
- Status: Published application
- Kind: A1
- Application number: 12/292,167
- Filed: 2008-11-13
- Published: 2009-08-27
- Earliest claimed: 2008-02-21
- Assignees: Hitachi, Ltd.
- Inventors: Tomoharu Kajiyama; Masataka Shirai; Hideki Kambara
- Web page: https://patentgrep.com/patent/US20090215162A1

## CPC

- B01L 3/50851: {specially adapted for heating or cooling samples}
- B01L 3/5025: {for parallel transport of multiple samples}
- B01L 3/50857: {using arrays or bundles of open capillaries for holding samples}
- B01L 2200/0668: Trapping microscopic beads
- B01L 2300/0819: Microarrays; Biochips
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

This invention provides a nucleic acid amplification device whereby the abundance of a target molecule can be maintained before and after a step of separately amplifying a sample such that highly accurate analysis results that can be applied to gene expression analysis can be obtained. Also, a nucleic acid amplification device having a structure in which a plurality of minute reaction cells each comprising a set of a bead-retaining space capable of retaining a single analysis bead and a reagent reaction space retaining no bead but having a volume that is large enough to cause a reagent reaction therein are positioned so as to form a planar face is provided.

## Claims

### Claim 1

A nucleic acid amplification device in which a plurality of reaction cells each comprising a set of a 1st space capable of retaining a single 1st bead and a 2nd space facing the 1st space are positioned so as to form a planar face, wherein the 1st space and the 2nd space are positioned in a manner such that the 1st bead is not located in a region in which the 1st space and the 2nd space do not overlap each other as viewed from the planar face.

### Claim 2 (depends on 1)

The nucleic acid amplification device according to claim 1, wherein the 2nd space does not retain a bead other than the 1st bead.

### Claim 3 (depends on 2)

The nucleic acid amplification device according to claim 2, wherein the following conditions 1) to 3) related to the diameter “r” of the 1st bead are satisfied:
1) the height of the 1st space is larger than “r/2” and smaller than “3r/2;”
2) the sum of the height of the 1st space and the height of a reagent-retaining space is “2r” or less; and
3) the diameter of the 1st space is larger than “r” and smaller than “2r.”

### Claim 4 (depends on 1)

The nucleic acid amplification device according to claim 1, wherein the 1st space and the 2nd space are connected to each other with a capillary having a diameter smaller than that of the 1st bead.

### Claim 5 (depends on 1)

The nucleic acid amplification device according to claim 1, wherein a flow cell is formed over the reaction cell.

### Claim 6 (depends on 5)

The nucleic acid amplification device according to claim 5, wherein the wall surface of a space (in the reaction cell) connected to the flow cell is inclined outward as viewed from the flow cell.

### Claim 7 (depends on 1)

The nucleic acid amplification device according to claim 1, comprising a member capable of separately forming each reaction cell that is installed on the opening of the reaction cell according to need.

### Claim 8 (depends on 7)

The nucleic acid amplification device according to claim 7, wherein the member has a layer made of an elastic material.

### Claim 9 (depends on 1)

The nucleic acid amplification device according to claim 1, wherein the 2nd space can retain a single 2nd bead that differs from the 1st bead.

### Claim 10 (depends on 9)

The nucleic acid amplification device according to claim 9, comprising a 3rd space, in which a reagent reaction is induced, between the 1st space and the 2nd space.

### Claim 11 (depends on 10)

The nucleic acid amplification device according to claim 10, wherein the following conditions related to the diameter “r” of the 1st bead are satisfied:
1) the heights of the 1st space retaining the 1st bead and the 2nd space are each larger than “r/2” and smaller than “3r/2;”
2) the sum of the height of the 1st or 2nd space and the height of the 3rd space is “2r” or less; and
3) the diameters of the 1st space and the 2nd space are each larger than “r” and smaller than “2r.”

### Claim 12 (depends on 9)

The nucleic acid amplification device according to claim 9, wherein the 1st space and the 2nd space are connected to each other with a capillary having a diameter smaller than that of the 1st bead.