Acetoin high-yield bacillus pumilus strain
AssigneeShanghai Apple Flavor & Fragrance Co., Ltd.
InventorsPing Xu, Zijun Xiao, Yi Du, Zhonghao Wei
Life of this patent
Estimating the term…
Abstract
A high-yield bacterial strain for producing acetoin named Bacillus pumilus XH195 has been deposited in a microorganism deposit center in Germany. The deposit number is DSM 16187. The bacteria of the strain are rod-shaped, 1.5 μm to 3.0 μm in length, and 0.6 μm to 0.7 μm in diameter. The colony color of the bacterial strain is yellow or white. The strain has the typical fatty acid profile of Bacillus and its physiological and biochemical characteristics align with those of Bacillus pumilus. When the bacteria of strain were cultured at 37° C. for 60 hours with shaking, the yield of acetoin could reach 63.0 g/L or 58.1 g/L, when glucose or sucrose was used as the substrate, respectively.
Claims10
-10. (canceled)
An isolated bacterial strain of Bacillus pumilus that grows on high osmotic LB medium and that produces a high yield of acetoin by fermentation of a sugar substrate.
The bacterial strain according to claim 1, wherein the bacterial strain exhibits one or more of the following characteristics:
- (a) rod-shape, length of 1.5-3.0 μm, and diameter of 0.6-0.7 μm;
- (b) a colony color of yellow or white;
- (c) a 16s rDNA sequence that is 98.7%-100% similar to the 16s rDNA of other Bacillus pumilus strains;
- (d) a typical fatty acid profile of Bacillus;
- (e) an ability to sporulate;
- (f) a positive VP reaction;
- (g) an ability to live in 10% NaCl solution (w/w) and grow under 50° C.;
- (h) an ability to produce acid when cultured with glucose, arabinose, xylose, mannitol, or fructose;
- (i) an ability to hydrolyze casein, gelatin, and Tween 80;
- and
- (j) an ability to use citrate as the carbon source.
A composition comprising a bacterial strain of Bacillus pumilus designated XH195, which is deposited as DSM 16187 in Deutsche Sammlung von Mikroorganismen und Zellkulturen.
The bacterial strain according to claim 1 wherein the high osmotic LB medium comprises (per liter distilled water) 200 g glucose, 10 g peptone, 5 g yeast extract, and 10 g NaC, and is sterilized at 121° C. for 15 min.
Dependencies are inferred from the claim text.