Method for Producing Virus-Type Particles Containing an Active Substance
Assigneeresponsif GmbH
InventorsChristoph Reichel, Claus Rühland, Jurgen Hess, Cristian Reiser
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Abstract
The invention relates to a method for producing virus-type particles containing an active substance. Proteins, which comprise a first amino acid sequence which is derived from a first virus protein, and fusion proteins are assembled in order to form the virus-type particles. The proteins and the fusion proteins are coexpressed in yeast cells.
Claims28
A method for producing virus-type particles containing an active substance, proteins each having a first amino acid sequence which is derived from a first virus protein and fusion proteins assemble to the virus-type particles, the first amino acid sequence being an amino acid sequence which is adequate for the formation of capsoid-forming capsomers that specifically bind a second virus protein, the fusion proteins having a second amino acid sequence each derived from the second virus protein and specifically binding to one of the capsomers each and a third amino acid sequence which forms the active substance, the proteins and the fusion proteins being co-expressed in yeast cells.
A method according to any of the preceding claims, the first virus protein being the virus protein 1 of the polyoma virus (VP1) and the second virus protein being the virus protein 2 (VP2) or the virus protein 3 (VP3) of the polyoma virus.
A method according to any of the preceding claims, the capsomers having the form of pentamers, hexamers or heptamers.
A method according to any of the preceding claims, the third amino acid sequence being, at least predominantly, hydrophobic.
A method according to any of the preceding claims, the third amino acid sequence forming the N terminal end of the fusion protein.
A method according to any of the preceding claims, wherein in the course of the co-expression of the proteins and of the fusion proteins the respective extent of the expression of the proteins and of the fusion proteins is harmonised in such a way that in the course of the method the biggest possible amount of the virus-type particles containing the active substance is formed.
A method according to any of the preceding claims, the third amino acid sequence comprising a sequence of at least one antigen, in particular a tumor-associated antigen, of at least one epitope of this antigen or of different epitopes of the said antigen.
A method according to any of the preceding claims, the third amino acid sequence forming an MHC-Class I-specific antigen.
A method according to any of the preceding claims, the yeast cells being yeast cells of the species Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, Hansenula polymorpha, Kluyveromyces lactis or Kluyveromyces marxianus.
Virus-type particles containing proteins each having a first amino acid sequence which is derived from a first virus protein, and fusion proteins, the first amino acid sequence being an amino acid sequence which is adequate for the formation of capsoid-forming capsomers which specifically bind a second virus protein, the fusion proteins having a second amino acid sequence each which is derived from the second virus protein and specifically binds to one of the capsomers each and a predominantly hydrophobic third amino acid sequence which forms an active substance.
Dependencies are inferred from the claim text.