Modified yeast consuming l-arabinose
AssigneeScandinavian Technology Group AB
InventorsEckhard Boles, Jessica Becker
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Abstract
The present invention relates to a method for producing a L-arabinose utilizing yeast strain for the production of ethanol, whereby a yeast strain is modified by introducing and expressing araA gene (L-arabinose isomerase), araB gene (L-ribulokinase D121-N) and araD gene (L-ribulose-5-P 4-epimerase) and carrying additional mutations in its genome or overexpressing a TAL1 (transaldolase) gene, enabling it to consume L-arabinose, to use it as the only carbon source, and to produce ethanol, as well as a method for producing ethanol using such a modified strain.
Claims15
A method for producing an L-arabinose utilizing Saccharomyces cerevisiae yeast strain for the production of ethanol, wherein a Saccharomyces cerevisiae CEN.PK strain is modified by introducing and expressing a B. subtilis araA gene (L-arabinose isomerase), an E. coli araB gene (L-ribulokinase) and an araD gene (L-ribulose-5-P 4-epimerase), and carrying additional mutations in its genome or overexpressing a TAL1 (transaldolase) gene, enabling it to consume L-arabinose, and to produce ethanol thereby from a medium comprising L-arabinose, whereby the araB gene is placed behind a weak promoter and the yeast strain is further modified by overexpressing the yeast GAL2 gene, and whereby the yeast strain is further modified by expressing a mutant form of the E. coli L-ribulokinase enzyme with reduced activity.
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Dependencies are inferred from the claim text.