Novel amide hydrolase gene
AssigneeMitsubishi Rayon Co., Ltd.
InventorsOsamu Katoh, Takanori Akiyama, Tetsuji Nakamura
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Abstract
The present invention relates to an amide hydrolase which is with excellent thermostability and stereoselectively hydrolyzes an α-amino acid amide; a gene encoding the enzyme protein; a novel recombinant vector containing the gene; a transformant containing the recombinant vector; and a process for producing an L-α-amino acid using the transformant.
Claims14
1-9. (canceled)
A protein of either (a) or (b):
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 1;
- or
- (b) a protein comprising the amino acid sequence of SEQ ID NO:
- 1, except that one to twenty amino acids have been deleted, replaced or added.
A polynucleotide encoding a protein of either (a) or (b):
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 1;
- (b) a protein comprising an amino acid sequence of SEQ ID NO:
- 1, except that one to twenty amino acids have been deleted, replaced or added.
A polynucleotide selected from (a), (b) or (c) comprising:
- (a) a polynucleotide consisting of the polynucleotide sequence of SEQ ID NO:
- 2;
- (b) a polynucleotide hybridizing under stringent conditions with the full complement of the polynucleotide sequence of SEQ ID NO:
- 2;
- wherein stringent conditions comprising washing in 2×SSC at 68° C.;
- or
- (c) a polynucleotide that is at least 95% homologous to SEQ ID NO:
- 2.
A microorganism belonging to genus Thermus or genus Bacillus, capable of growing at 55° C. or higher, and producing an amide hydrolase with excellent thermostability.
Dependencies are inferred from the claim text.