Sialyltransferases comprising conserved sequence motifs
AssigneeNational Research Council of Canada
InventorsMichel Gilbert, Warren W.. Wakarchuk
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Abstract
The present invention provides, e.g., sialyltransferase proteins comprising conserved sequence motifs, including α-2,3-sialyltransferase proteins from C. jejuni strains O:36 and O:19. The invention also provides methods of making sialylated products using those sialyltransferases.
Claims42
An isolated or recombinant sialyltransferase polypeptide;
- wherein the sialyltransferase polypeptide transfers sialic acid from a donor substrate to an acceptor substrate;
- and wherein the sialyltransferase polypeptide comprises a sialyltransferase motif A and a sialyltransferase motif B;
- with the proviso that the sialytransferase polypeptide is not a member of the group selected from GenBank AF130466, GenBank AX934425, GenBank AX934434, GenBank AX934427, GenBank AX934431, GenBank AF401529, GenBank AX934436, GenBank AX934429, GenBank AY044156, GenBank AF400047, GenBank AY297047, GenBank AF305571, GenBank AL139077, GenBank X57315, GenBank AE006157, SEQ ID NO:
- 43 through SEQ ID NO:57 and a consensus sequence of a sialyltransferase protein derived from CD:
- pfam06002.2, CST-I, (SEQ ID NO:32).
An isolated or recombinant sialyltransferase polypeptide;
- wherein the sialyltransferase polypeptide transfers sialic acid from a donor substrate to an acceptor substrate;
- and
- wherein the sialyltransferase polypeptide comprises an amino acid sequence with at least 98% identity to the amino acid sequence of FIG. 2 (O:36 amino acid sequence, SEQ ID NO:6).
An isolated or recombinant sialyltransferase polypeptide;
- wherein the sialyltransferase polypeptide transfers sialic acid from a donor substrate to an acceptor substrate;
- and
- wherein the sialyltransferase polypeptide comprises amino acids 1-283 of the amino acid sequence of FIG. 2 (O:36 amino acid sequence, SEQ ID NO:6).
An isolated or recombinant sialyltransferase polypeptide;
- wherein the sialyltransferase polypeptide transfers sialic acid from a donor substrate to an acceptor substrate;
- and
- wherein the sialyltransferase polypeptide comprises amino acids 1-285 of the amino acid sequence of FIG. 3 (O:19 amino acid sequence, SEQ ID NO:8)
An isolated or recombinant nucleic acid that comprises a sialyltransferase polynucleotide sequence that encodes a sialyltransferase polypeptide;
- wherein the sialyltransferase polypeptide transfers sialic acid from a donor substrate to an acceptor substrate;
- and
- wherein the sialyltransferase polynucleotide sequence comprises a nucleotide sequence with at least 98% identity to the nucleic acid sequence of FIG. 2 (O:36 nucleic acid sequence, SEQ ID NO:5).
An isolated or recombinant nucleic acid that comprises a sialyltransferase polynucleotide sequence that encodes a sialyltransferase polypeptide;
- wherein the sialyltransferase polypeptide transfers sialic acid from a donor substrate to an acceptor substrate;
- and
- wherein the sialyltransferase polynucleotide sequence comprises nucleotides I-849 of FIG. 2 (O:36 nucleic acid sequence, SEQ ID NO:5).
An isolated or recombinant nucleic acid that comprises a sialyltransferase polynucleotide sequence that encodes a sialyltransferase polypeptide;
- wherein the sialyltransferase polypeptide transfers sialic acid from a donor substrate to an acceptor substrate;
- and
- wherein the sialyltransferase polypeptide comprises nucleotides 1-888 of FIG. 3 (O:19 nucleic acid sequence, SEQ ID NO:7).
An isolated or recombinant nucleic acid that comprises a sialyltransferase polynucleotide sequence that encodes a sialyltransferase polypeptide;
- wherein the sialyltransferase polypeptide transfers sialic acid from a donor substrate to an acceptor substrate;
- and
- wherein the sialyltransferase polypeptide comprises amino acids 1-285 of the amino acid sequence of FIG. 2 (O:36 amino acid sequence, SEQ ID NO:6).
An isolated or recombinant nucleic acid that comprises a sialyltransferase polynucleotide sequence that encodes a sialyltransferase polypeptide;
- wherein the sialyltransferase polypeptide transfers sialic acid from a donor substrate to an acceptor substrate;
- and
- wherein the sialyltransferase polypeptide comprises amino acids 1-285 of the amino acid sequence of FIG. 3 (O:19 amino acid sequence, SEQ ID NO:8).
Dependencies are inferred from the claim text.