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US 2009/0215111 A1Published application
Analysis of steroid hormones in thin tissue sections
OwnerTimothy D. Veenstra, Xia Xu +3inventors
InventorsTimothy D. Veenstra, Xia Xu, Josip Blonder, Donald J. Johann, JR., Regina G. Ziegler
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Abstract
Mass-spectrometry based methods of analyzing estrogens and other steroids from biological tissue sections samples are disclosed herein. Methods of detecting a disease state or condition or elevated risk of a disease state or condition in a mammal from tissue sections are also disclosed.
Claims33
1-71. (canceled)
A method of quantifying steroid hormone composition in tissue, comprising;
- preparing tissue into one or more micron-scale thick tissue sections;
- lysing at least a portion of the micron-scale tissue sections to form one or more lysed sections;
- extracting at least a portion of the lysate from at least one of the lysed sections using solvent to form one or more extracted lysates;
- derivatizing at least a portion of the one or more extracted lysates to form one or more derivatized lysates;
- and
- analyzing at least a portion of the one or more derivatized lysates using a molecular mass analysis system to quantifying the steroid hormone composition in the tissue.
3 more elements
A method of detecting a disease state or condition in a mammal, comprising:
- preparing tissue into one or more micron-scale thick tissue sections;
- lysing at least a portion of the one or more tissue sections to form one or more lysed sections;
- extracting at least a portion of the lysate from one or more of the lysed sections to form one or more extracted lysates;
- derivatizing at least a portion of the one or more extracted lysates to form one or more derivatized lysates;
- analyzing at least a portion of the one or more derivatized lysates by mass spectrometry to provide a steroid metabolite profile of one or more of the micron-scale thick tissue sections;
- and
- comparing the steroid metabolite profile of the one or more micron-scale thick tissue sections to one or more steroid metabolite profiles.
4 more elements
Dependencies are inferred from the claim text.