Selecting, culturing and creating lineage committed hematopoietic stem cells
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsSusan Kaye Nilsson, David Norman Haylock
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Abstract
The present invention provides a method for selecting hematopoietic stem cells (HSCs) comprising providing an agent which binds to α9β1 integrin on the cell surface to a population of cells including HSCs and separating HSCs by virtue of the binding agent. The invention also provides a method of culturing a population of HSCs in the presence of an agent which binds to α9β1, wherein the agent inhibits differentiation of the HSCs. The invention also provides a method of producing a population of lineage committed cells comprising culturing HSCs in the presence of an agent which inhibits or prevents binding to α9β1.
Claims12
A method for selecting HSCs comprising:
- (a) providing a starting population of cells comprising HSCs;
- and
- (b) selecting HSCs within the population by providing an agent that binds to α9β1 integrin on the cell surface of the HSCs;
- and
- (c) separating HSCs from the starting population of cells by virtue of the binding of the agent.
A method for producing a self-renewable population of HSCs comprising:
- (a) providing a starting population of cells enriched in HSCs;
- (b) culturing HSCs in the presence of an agent which binds to α9β1 on the cell surface of HSCs wherein the agent inhibits differentiation of the self-renewable HSCs;
- and
- (c) harvesting said population of self-renewable HSCs.
A method for producing a population of lineage committed cells comprising:
- (a) providing a starting population of cells enriched in HSCs;
- (b) culturing HSCs in the presence of an agent which inhibits or prevents binding to α9β1 integrin present on the cell surface of HSCs, wherein said inhibition increases overall proliferation and differentiation of HSCs to produce lineage committed cells;
- and
- (c) harvesting said lineage committed cells.
Dependencies are inferred from the claim text.