Click chemistry surface functionalization for resonant micro-cavity sensors
AssigneeCalifornia Institute of Technology
InventorsAndrea M. Armani, Akinleye C. Alabi, Mark E. Davis, Richard C. Flagan, Scott E. Fraser
Life of this patent
Estimating the term…
Abstract
Micro-cavity resonant sensors have outer surfaces that are functionalized using click chemistry, e.g., involving a cycloaddition reaction of an alkyne functional group and an azide functional group. A first polymer linking element binds to an outer surface of the micro-cavity and has an azide functional group, which bonds to an alkyne functional group of a second polymer linking element as a result of a cycloaddition reaction. A functionalization element such as an antibody, antigen or protein for sensing a target molecule is bound to the second linking element.
Claims21
A method for preparing a resonant micro-cavity for use as a sensor, comprising:
- functionalizing an outer surface of the surface of the resonant micro-cavity utilizing click chemistry.
A method for functionalizing a resonant micro-cavity for use as a sensor, comprising:
- introducing a first linking element into a solution in which the resonant micro-cavity is placed, the first linking element binding to an outer surface of the resonant micro-cavity and having a first functional group;
- introducing a second linking element into the solution, the second linking element having a second functional group, wherein a bond forms between the first functional group and second functional group as a result of a cycloaddition reaction;
- and
- introducing a functionalization element for sensing a target molecule into the solution, the functionalization element bonding to the second linking element.
A method for functionalizing a resonant micro-cavity for use as a sensor, comprising:
- introducing a first polymer linking element having a molecular weight greater than 100 into a solution in which the resonant micro-cavity is placed, the first polymer linking element binding to an outer surface of the resonant micro-cavity and having an azide functional group;
- introducing a second polymer linking element having a molecular weight greater than 100 into the solution, the second polymer linking element having an alkyne functional group that bonds to the azide functional group as a result of a cycloaddition reaction;
- and
- introducing a functionalization element for sensing a target molecule into the solution, the functionalization element bonding to the second linking element.
21-65. (canceled)
Dependencies are inferred from the claim text.