Nanoparticles with lipid core and polymer shell structures for protein drug delivery prepared by nanoencapsulation

A method for preparing nanoparticles with a lipid core and polymer shell structures for protein drug delivery comprising:

• a) sonicating lecithin as represented by formula 1 to form nano-sized particulate lipids (nanolipids);
• b) mixing the nanolipids and a protein drug;
• c) adding the above-prepared mixture of the nanolipids and a protein drug to an aqueous solution comprising triblock copolymer as represented by formula the 2 and a cryoprotectant to form a homogenous solution;
• and
• d) freeze-drying the solution obtained in step c).

A method for preparing nanoparticles with a lipid core and polymer shell structures for protein drug delivery, wherein a protein drug has positive charges, comprising:

• a) sonicating lecithin as represented by the formula 1 to form nano-sized particulate lipids (nanolipids);
• b) adding a cryoprotectant to an aqueous solution comprising the nanolipids obtained in step a);
• c) mixing the above-prepared solution comprising the nanolipids and a cryorotectant with an aqueous solution comprising triblock copolymer as represented by the formula 2 and a protein drug having positive charge;
• and
• d) freeze-drying the solution obtained in step c).

A method for preparing nanoparticles with a lipid core and polymer shell structures for protein drug delivery, wherein a protein drug has negative charge, comprising:

• a) sonicating lecithin as represented by the formula 1 to form nano-sized particulate lipids (nanolipids);
• b) mixing the nanolipids, a protein drug having negative charge and low-molecular-weight chitosan;
• c) adding the mixture of nanolipids, a protein drug having negative charge and low-molecular-weight chitosan to an aqueous solution comprising triblock copolymer as represented by the formula 2 and a cryoprotectant to form a homogenous solution;
• and
• d) freeze-drying the solution obtained in step c).