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AssigneeFundacion Para La Investigacion Biomedica Del Hospital Gregorio Maranon
InventorsJose Maria Lasso Vazquez, Paola Nava Perez
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The invention relates to an endothelized artificial matrix comprising a fibrin gel, which is a superproducer of proantiogenic factors. The inventive matrix comprises a fibrin gel containing embedded endothelial cells which have been transfected in vitro with at least one adenoviral vector containing the sequence encoding at least one proangiogenic factor, which is inserted such that it can be overexpressed in said endothelial cells. The insertion of the aforementioned matrix between a flap and the receptor site thereof during a transplant procedure improves the survival rates of said flap, since the endothelized matrix can induce angiogenesis both in the flap and in the receptor site and, in this way, improve the vascularization of the transplanted area.
An artificial matrix of an endothelialised fibrin gel which contains endothelial cells embedded in its interior that, in part or in its entirety, has been transfected in vitro with one or more adenoviral vectors which has in its sequence at least one gene corresponding to a proangiogenic factor capable of overexpression in the aforementioned transfected endothelial cells.
A method for obtaining an artificial matrix of a superproducer endothelialised fibrin gel of at least one proangiogenic factor, which comprises the following steps:
In an operation to implant a tissue flap in a mammal, a method of producing a vascularized bridge between said flap and tissue of a recipient thereof which comprises inserting a matrix of a superproducer endothelialised fibrin gel of at least one proangiogenic factor between said flap and a receptor site therefor wherein said matrix is an artificial matrix of an endothelialised fibrin gel which contains endothelial cells embedded in its interior that, in part or in its entirety, has been transfected in vitro with one or more adenoviral vectors which has in its sequence at least one gene corresponding to a proangiogenic factor capable of overexpression in the aforementioned transfected endothelial cells.
Dependencies are inferred from the claim text.