Means for Obtaining Avirulent Leishmania Promastigotes, Promastigotes Obtained, and the Applications Thereof
AssigneeInstitut De Recherche Pour Le Developpement (Ird)
InventorsJean-Loup Lemesre, Philippe Holzmuller, Rachel Bras-Goncalves
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Abstract
The invention relates to an expression vector of a gene coding for an antigenic protein of Leishmania promastigote, characterised in that it comprises a PSA gene insert in opposite orientation. The invention can be applied to the development of mutants under-expressing, or no longer expressing, genes coding for an antigenic protein of Leishmania promastigote, and to the therapeutic and/or vaccine-oriented uses thereof.
Claims8
An expression vector for a gene encoding an antigenic leishmania promastigote protein, characterized in that it comprises an insert of a PSA gene in an antisense orientation.
A method of obtaining avirulent forms of promastigotes, characterized in that it comprises:
- nondirectional insertion, into a leishmania expression vector, of genes encoding antigenic leishmania peptides or proteins,
- selection of the vectors containing the inserts in an antisense orientation,
- transfection of leishmania promastigote forms using the selected vectors,
- biological cloning of the genetically modified promastigotes by micromanipulation so as to obtain mutants which underexpress, or even which no longer express, the gene encoding an antigenic leishmania promastigote protein.
Dependencies are inferred from the claim text.