Peptide mixtures with immunomodulatory activity
OwnerRichard A. Houghten, Clemencia Pinilla +4inventors
InventorsRichard A. Houghten, Clemencia Pinilla, Roland Martin, Claus-Steffen Sturzebecher, Jacquline Shukaiak-Quant, Henry F. McFarland
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Abstract
The present invention is related to complex peptide mixtures with immunomodulatory effects and methods for their use. The invention is further directed toward treatments of diseases through the modulation of the immune system with complex peptide mixtures. The invention is also directed to methods for improving the characteristics of complex peptide mixtures.
Claims58
1-84. (canceled)
A complex peptide mixture, comprising a plurality of peptides having a length within the range of 8 to 20 amino acids, wherein said mixture comprises peptides having a degree of diversity at defined positions in the peptide chain, wherein the degree of diversity in at least one defined position is different from the degree of diversity in at least one other defined position, and wherein in a majority of the mixture, the peptides include in at least four positions all of A, E, K, and Y and no other amino acids.
A complex peptide mixture, comprising a plurality of peptides having a length within the range of 8 to 20 amino acids, wherein said mixture comprises peptides having a degree of diversity at defined positions in the peptide chain and wherein at least in a majority of the mixture, the amino acids in the peptides include all of the following specified amino acids and no other amino acids:
- in at least four positions:
- A, E, K, and Y;
- in at least one position:
- I, L, and V;
- in at least one position:
- H, R, and K;
- and
- in at least one position:
- P and I.
A method of treating a disease, comprising administering to a vertebrate a complex peptide mixture, wherein said mixture comprises peptides having a constrained degree of diversity at each of at least 4 defined positions in the peptide chain and wherein the constrained degree of diversity in at least one defined position is different from the constrained degree of diversity in at least one other defined position.
A method for stimulating an immune cell comprising administration of a complex peptide mixture to said cell, wherein said mixture comprises peptides having a constrained degree of diversity at each of at least 4 defined positions in the peptide chain, and wherein the degree of diversity in at least one defined position is different from the degree of diversity in at least one other defined position.
A method for suppression of an immune reaction to an antigen, comprising administering a complex peptide mixture to an individual, said complex peptide mixture comprising peptides having a constrained degree of diversity at each of at least 10 defined positions in the peptide chain, and wherein the degree of diversity in at least one or two defined positions is different from the degree of diversity in at least one or two other defined positions.
A method for inducing an anti-inflammatory response from immune system cells comprising administering a complex peptide mixture to immune system cells, said complex peptide mixture comprising peptides having a constrained degree of diversity at each of at least 10 defined positions in the peptide chain, and wherein the constrained degree of diversity in at least one or two said defined positions is different from the degree of diversity in at least another defined position.
A method for creating a high affinity peptide ligand of a defined formula for a receptor of a T cell that is reactive to a myelin antigen, copolymer or an active mixture, comprising the deconvolution of a complex peptide mixture of a formula selected from the group consisting of the formulas listed in Table 1.
A complex peptide mixture, wherein said mixture comprises peptides between 4 and 100 amino acid residues in length, wherein said mixture has a constrained degree of diversity at each of at least 10 defined positions in the peptide chain, and wherein the degree of diversity in at least one defined position is different from the degree of diversity in at least one other defined position.
A method of enhancing a biological property of a complex mixture of peptides, peptidomimetics, or peptides and peptidomimetics, comprising:
- providing a set of complex mixtures of reduced complexity that have a reduced degree of diversity from said complex mixture at least at one position;
- testing each of said complex mixtures of reduced complexity and said complex mixture in an assay;
- identifying complex mixtures of reduced complexity that have a greater or lesser activity in said assay than said complex mixture;
- and
- enhancing the biological property of said complex mixture by incorporating the complexity-reducing features of at least one of said complex mixtures of reduced complexity that have greater or lesser activity in said assay than said complex mixture into the formula of said complex peptide mixture.
A complex peptide mixture, comprising a plurality of peptides having a length within the range of 8 to 20 amino acids, wherein said mixture comprises peptides having a degree of diversity at defined positions in the peptide chain, and wherein at least in a majority of the mixture, the identity of 10 contiguous amino acids in the peptides are defined by the following formulas:
- FW-EF-EK-AEK-AKY-ANY-ANY-AINV-ASV-Y;
- or EFWY-EFIVWY-EFKQ-AEKQ-AKQY-ANQY-AGNSY-AGINSV-AIQSV-IKRSVY.
A complex peptide mixture, comprising a plurality of peptides having a length within the range of 8 to 20 amino acids, wherein said mixture comprises peptides having a degree of diversity at defined positions in the peptide chain, wherein the degree of diversity in at least one defined position is different from the degree of diversity in at least one other defined position, wherein in a majority of the mixture, the peptides include in at least one but no more than five positions all of A, E, K, and Y and no other amino acids.
Dependencies are inferred from the claim text.