Colon cancer related gene tom34
AssigneesOncotherapy Science, Inc., The University of Tokyo
InventorsYusuke Nakamura, Youchi Furukawa, Hideaki Tahara, Takuya Tsunoda, Satoshi Matsushima
Life of this patent
Estimating the term…
Abstract
Objective methods for detecting and diagnosing colon cancer are described herein. In one embodiment, the diagnostic method involves determining the expression level of TOM34 that discriminates between colon cancer cells and normal cells. Finally, the present invention provides methods of screening for therapeutic agents useful in the treatment of colon cancer, methods of treating colon cancer and method for vaccinating a subject against colon cancer.
Claims33
A method of diagnosing colon cancer or a predisposition for developing colon cancer in a subject, comprising determining a level of expression of TOM34 in a patient-derived biological sample, wherein an increase in said sample expression level as compared to a normal control level of said gene indicates that said subject suffers from or is at risk of developing colon cancer.
A method of screening for a compound for treating or preventing colon cancer, said method comprising the steps of:
- a) contacting a test compound with a polypeptide encoded by a polynucleotide of TOM34;
- b) detecting the binding activity between the polypeptide and the test compound;
- and
- c) selecting the test compound that binds to the polypeptide.
A method of screening for a compound for treating or preventing colon cancer, said method comprising the steps of:
- a) contacting a candidate compound with a cell expressing TOM34;
- and
- b) selecting the candidate compound that reduces the expression level of TOM34 as compared to the expression level of TOM34 detected in the absence of the test compound.
A method of screening for a compound for treating or preventing colon cancer, said method comprising the steps of:
- a) contacting a test compound with a polypeptide encoded by a polynucleotide of TOM34;
- b) detecting the biological activity of the polypeptide of step (a);
- and
- c) selecting the test compound that suppresses the biological activity of the polypeptide encoded by the polynucleotide of TOM34 as compared to the biological activity of said polypeptide detected in the absence of the test compound.
A method of screening for compound for treating or preventing colon cancer, said method comprising the steps of:
- a) contacting a candidate compound with a cell into which a vector, comprising the transcriptional regulatory region of TOM34 and a reporter gene that is expressed under the control of the transcriptional regulatory region, has been introduced;
- b) measuring the expression or activity of said reporter gene;
- and
- c) selecting the candidate compound that reduces the expression or activity of said reporter gene.
A kit comprising a detection reagent which binds to (a) TOM34, or (b) polypeptides encoded by TOM34.
A method of treating or preventing colon cancer in a subject comprising administering to said subject an antisense composition, said antisense composition comprising a nucleotide sequence complementary to a coding sequence of TOM34.
A method of treating or preventing colon cancer in a subject comprising administering to said subject an siRNA composition, wherein said siRNA composition reduces the expression of TOM34.
A method of treating or preventing colon cancer in a subject comprising the step of administering to said subject a pharmaceutically effective amount of an antibody, or immunologically active fragment thereof, that binds to a protein of TOM34.
A method of treating or preventing colon cancer in a subject comprising administering to said subject a vaccine comprising (a) a polypeptide encoded by a nucleic acid of TOM34, (b) an immunologically active fragment of said polypeptide, or (c) a polynucleotide encoding the polypeptide or said immunologically active fragment of (b).
A method of inducing an anti-tumor immunity, said method comprising the step of contacting with an antigen presenting cell a polypeptide, a polynucleotide encoding the polypeptide or a vector comprising the polynucleotide, wherein the polypeptide is encoded by TOM34 or an immunologically active fragment of said polypeptide.
(canceled)
A composition for treating or preventing colon cancer, said composition comprising a pharmaceutically effective amount of an antisense polynucleotide or siRNA against a polynucleotide of TOM34.
A composition for treating or preventing colon cancer, said composition comprising a pharmaceutically effective amount of an antibody or fragment thereof that binds to a protein encoded by TOM34.
(canceled)
A decapeptide comprising the amino acid sequence of SEQ ID NO:
- 7.
A peptide having cytotoxic T cell inducibility, wherein the peptide comprises the amino acid sequence of SEQ ID NO:
- 7, wherein zero, 1, 2, or several amino acids are substituted or added.
Dependencies are inferred from the claim text.