Methods and Compositions for Treating and Preventing Bacterial Infections
OwnerJohn Mekalanos, Stefan Pukatzki +1inventors
InventorsJohn Mekalanos, Stefan Pukatzki, Joseph Mougous
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Abstract
The invention features methods and compositions for treating or preventing Gram-negative bacterial infections.
Claims44
A method of treating or preventing a Gram-negative bacterial infection in a patient, said method comprising the steps of providing a humanized or human antibody or antibody fragment directed to a polypeptide selected from Hcp, VgrG, and Saf, and systemically administering the antibody to the patient, wherein the antibody treats or prevents Gram-negative bacterial infection.
A method of treating or preventing Pseudomonas aeruginosa infection in a patient comprising the steps of providing a humanized or human antibody or antibody fragment directed to a polypeptide selected from Hcp, VgrG1, VgrG2, VgrG3, SafA, SafB, and SafC, and administering the antibody to the lungs of the patient.
An antibody specific for a Gram-negative bacterium polypeptide selected from Hcp, VgrG, and Saf.
A method of inhibiting infection of a Gram-negative bacterium in a patient in need thereof, said method comprising administering to the patient an effective amount of an Hcp, VgrG, or Saf antigen.
A method of preventing or treating a Gram negative bacterial infection in a patient, said method comprising administering an effective amount of a compound that inhibits secretion or activity of Hcp or VgrG.
An attenuated bacterial mutant, wherein said attenuated mutant contains a mutation of a gene of an IAHP locus.
A method for identifying an antimicrobial drug, said method comprising:
- (a) contacting a candidate compound and a polypeptide encoded by a gene of an IAHP locus;
- and (b) comparing the biological activity of said polypeptide in the presence and absence of said candidate compound, wherein alteration of the biological activity of said polypeptide indicates that said candidate compound is an antimicrobial drug.
A method for identifying an antimicrobial drug, said method comprising:
- (a) contacting a candidate compound and a polypeptide encoded by a gene of an IAHP locus;
- and (b) detecting binding of said candidate compound and said polypeptide, wherein binding indicates that said candidate compound is an antimicrobial drug.
A method for identifying an antimicrobial drug, said method comprising:
- (a) contacting a candidate compound and a Gram negative bacterium;
- and (b) detecting secretion of Hcp or VgrG, wherein a decrease in secretion, relative to secretion by said Gram negative bacterium not contacted with said candidate compound, indicates that said candidate compound is an antimicrobial drug.
Dependencies are inferred from the claim text.