Cancer Vaccines and Therapeutic Methods
AssigneeThe Board of Trustees of the University of Illinoi
InventorEdward P. Cohen
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Abstract
Compositions and methods of producing improved cancer vaccines are described. In addition, methods of identifying tumor associated antigens are also described.
Claims38
A composition comprising a chemotherapeutic agent and an antigen-presenting cell modified to express an allogeneic MHC-determinant, and wherein the cell has been transfected with genomic DNA or c-DNA isolated from the tumor of a mammal.
A method of treating a tumor in a mammal, comprising administering to the mammal a chemotherapeutic agent and an effective amount of an antigen-presenting cell modified to express an allogeneic MHC-determinant, and wherein the cell has been transfected with genomic DNA isolated from the tumor of the mammal.
A method of treating a tumor in a mammal, comprising administering to the mammal a chemotherapeutic agent and an effective amount of an antigen-presenting cell that has been transfected with genomic DNA or c-DNA isolated from the tumor of the mammal.
A method of enriching a population of immunogenic cells capable of inducing an immune response to a target cell in a patient, comprising:
- (a) providing a population of immunogenic cells capable of inducing an immune response to a target cell in a patient;
- (b) incubating the immunogenic cells in growth medium;
- (c) either before or after (b), diluting the suspension the immunogenic cells, whereby an enriched population of immunogenic cells is produced;
- and
- (d) optionally repeating (a)-(c).
A method of identifying a tumor associated antigen, comprising:
- (a) providing a recipient cell and an immunogenic cell capable of inducing an immune response to a tumor cell;
- and
- (b) comparing the nucleic acid of the recipient cell and the immunogenic cell, whereby a nucleic acid with increased levels in the immunogenic cell encodes a tumor associated antigen.
A method of identifying a tumor associated antigen, comprising:
- (a) providing a recipient cell and an immunogenic cell capable of inducing an immune response to a tumor cell;
- and
- (b) comparing the expressed proteins of the immunogenic cell and the recipient cell, whereby a protein with increased expression in the immunogenic cell is a tumor associated antigen.
A composition comprising an antigen-presenting cell modified to express an allogeneic MHC-determinant, and wherein the cell has been transfected with genomic DNA or c-DNA isolated from the tumor of a mammal.
Dependencies are inferred from the claim text.