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US 2009/0214490 A1Published application
Human embryonic stem cell methods and podxl expression
AssigneeAgency for Science, Technology and Research
InventorAndre Choo
Life of this patent
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AssigneeAgency for Science, Technology and Research
InventorAndre Choo
Estimating the term…
A method of identifying an undifferentiated human embryonic stem cell in a sample which may contain such cells, the method comprising identifying the cell or cells within the sample that express podocalyxin-like protein (PODXL) on their surface. A method of isolating an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising isolating the cell or cells within the sample that express PODXL on their surface. Typically, the methods use an antibody which binds to PODXL. Undifferentiated human embryonic stem cells isolated by the method may be useful in cell therapy. Also, in particular, compositions of cells differentiated from a human embryonic stem cell but which composition has been depleted of undifferentiated human embryonic stem cells are provided which are useful in cell therapy.
A method of identifying an undifferentiated human embryonic stem cell in a sample which may contain such cells, the method comprising identifying the cell or cells within the sample that express podocalyxin-like protein (PODXL) on their surface.
A method of isolating an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising isolating the cell or cells within the sample that express PODXL on their surface.
A method of removing an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising removing from the sample the cell or cells that express PODXL on their surface.
A method of destroying an undifferentiated human embryonic stem cell in a sample containing such cells, the method comprising destroying the cell or cells in the sample that express PODXL on their surface.
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A moiety which binds to PODXL further comprising a detectable label.
A moiety which binds to PODXL further comprising a cytotoxic moiety.
A kit of parts comprising a moiety which binds PODXL and a further agent that detects another human embryonic stem cell marker.
An isolated undifferentiated human embryonic stem cell which expresses PODXL on its surface.
A composition containing cells differentiated from undifferentiated human embryonic stem cells which composition contains substantially no undifferentiated human embryonic stem cells which express PODXL on their surface.
A method of treating a patient in need of cell therapy, the method comprising administering to the patient an undifferentiated human embryonic cell obtained by isolating an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising isolating the cell or cells within the sample that express PODXL on their surface or an isolated undifferentiated human embryonic stem cell which expresses PODXL on its surface or a composition containing differentiated cells but which is depleted in undifferentiated human embryonic stem cells obtained by a method of removing an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising removing from the sample the cell or cells that express PODXL on their surface or a method of destroying an undifferentiated human embryonic stem cell in a sample containing such cells, the method comprising destroying the cell or cells in the sample that express PODXL on their surface or a composition containing cells differentiated from undifferentiated human embryonic stem cells which composition contains substantially no undifferentiated human embryonic stem cells which express PODXL on their surface.
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A method of making a pharmaceutical composition of undifferentiated human embryonic stem cells, the method comprising isolating undifferentiated human embryonic stem cells from a sample containing such cells, the method comprising isolating the cell or cells within the sample that express PODXL on their surface or providing isolated undifferentiated human embryonic stem cells which expresses PODXL on their surface and incorporating the cells so isolated into a pharmaceutical composition.
A method of making a pharmaceutical composition containing differentiated cells but which is depleted of undifferentiated human embryonic stem cells, the method comprising removing an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising removing from the sample the cell or cells that express PODXL on their surface or destroying an undifferentiated human embryonic stem cell in a sample containing such cells, the method comprising destroying the cell or cells in the sample that express PODXL on their surface, wherein the sample is a sample in which undifferentiated human embryonic stem cells have been encouraged to differentiate into particular cell lineages, and the sample contains differentiated cells derived from undifferentiated human embryonic stem cells, or providing a composition containing differentiated cells but which is depleted in undifferentiated human embryonic stem cells obtained by a method of removing an undifferentiated human embryonic stem cell from a sample containing such cells, the method comprising removing from the sample the cell or cells that express PODXL on their surface or a method of destroying an undifferentiated human embryonic stem cell in a sample containing such cells, the method comprising destroying the cell or cells in the sample that express PODXL on their surface, and incorporating said composition of cells into a pharmaceutical composition.
An anti-PODXL antibody that contains the amino acid sequences i) to iii), or the amino acid sequences iv) to vi), or preferably the amino acid sequences i) to vi):
An antibody that selectively binds to the PODXL epitope bound by an antibody having at least one light chain variable region comprising the amino acid sequence set out in FIG. 11 (SEQ ID NO:
Dependencies are inferred from the claim text.