Novel mannose-specific adhesins and their use
OwnerGabriele Pretzer, Johannes Snel +3inventors
InventorsGabriele Pretzer, Johannes Snel, Peter Allard Bron, Willem Meindert De Vos, Michiel Kleerebezem
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel mannose-specific adhesins, and variants thereof, as well as nucleic acid sequences encoding these. The invention also relates to host cells expressing nucleic acids encoding the mannose-specific adhesins or variants thereof, as well as to pharmaceutical or nutraceutical compositions comprising the mannose-specific adhesins and/or host cells expressing the adhesins, and their use in treating or preventing bacterial infection. Further, screening methods suitable for identifying bacteria strains with probiotic properties are provided.
Claims24
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a mannose-specific adhesin, whereby the nucleotide sequence is selected from the group consisting of:
- (a) nucleotide sequences encoding a polypeptide comprising an amino acid sequence that has at least 60% sequence identity with the amino acid sequence of SEQ ID NO. 1;
- (b) nucleotide sequences comprising a nucleotide sequence that has at least 60% sequence identity with the nucleotide sequence of SEQ ID NO. 2;
- (c) nucleotide sequences the complementary strand of which hybridises to a nucleic acid molecule sequence of (a) or (b);
- (d) nucleotide sequences the sequence of which differs from the sequence of a nucleic acid molecule of (c) due to the degeneracy of the genetic code.
An isolated polypeptide having an amino acid sequence that has at least 60% sequence identity with the amino acid sequence of SEQ ID NO. 1.
A method for producing a polypeptide, the method comprising the steps of:
- a) providing a host cell comprising a nucleotide sequences operably linked to a DNA regulatory element, whereby said nucleotide sequence encodes an amino acid sequence that has at least 60% sequence identity with the amino acid sequence of SEQ ID NO. 1,
- b) culturing a said host cell under conditions conducive to the expression of the polypeptide and,
- c) recovery of the polypeptide.
A method for producing a composition, the method comprising the steps of:
- a) providing a host cell comprising a nucleotide sequences operably linked to a DNA regulatory element, whereby said nucleotide sequence encodes an amino acid sequence that has at least 60% sequence identity with the amino acid sequence of SEQ ID NO. 1,
- b) culturing said host cell under conditions conducive to the expression of a polypeptide,
- c) recovery of the polypeptide, and
- d) mixing the polypeptide with a pharmaceutically or physiologically acceptable carrier.
A method for producing a composition, the method comprising the steps of:
- a) providing a host cell comprising a nucleotide sequence operably linked to a DNA regulatory element, whereby said nucleotide sequence encodes an amino acid sequence that has at least 60% sequence identity with the amino acid sequence of SEQ ID NO. 1,
- b) culturing a said host cell;
- and,
- c) mixing the host cell with a pharmaceutically or physiologically acceptable carrier.
Dependencies are inferred from the claim text.