Latent phase viral interleukin-10-(VII-10) and uses thereof
AssigneeThe University of Sydney
InventorsBarry Slobedman, Allison Denise Abendroth, Christina Anne Jenkins
Life of this patent
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Abstract
The present invention relates to a purified nucleic acid sequence encoding a homologue of human interleukin 10 (IL-10), wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group. The present invention also relates to uses of this polypeptide, in particular for diagnosing disease states and screening for modulator and inhibitor compounds of such polypeptides and in turn the virus itself, screening for infection in vertebrates and biological tissue, cleansing of infected biological tissues, and in the treatment and/or prophylaxis and/or diagnosis of disease caused by a virus of the herpesvirideae group.
Claims57
A purified nucleic acid sequence encoding a homologue of human interleukin 10 (IL-10), wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group, and wherein said nucleic acid sequence is as set forth in SEQ ID NO:1.
An isolated homologue of human interleukin 10 (IL-10) polypeptide, wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group, and wherein said IL-10 homologue has the amino acid sequence as set forth in SEQ ID NO:10, or the amino acid sequence as set forth in SEQ ID NO:10 including one or more conservative amino acid substitutions.
A vector comprising a nucleic acid sequence encoding an isolated homologue of human interleukin 10 (IL-10) polypeptide, wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group, and wherein said IL-10 homologue has the amino acid sequence as set forth in SEQ ID NO:10, or the amino acid sequence as set forth in SEQ ID NO:10 including one or more conservative amino acid substitutions.
A kit comprising a purified nucleic acid sequence encoding a homologue of human interleukin 10 (IL-10), wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group, and wherein said nucleic acid sequence is as set forth in SEQ ID NO:1 or an isolated homologue of human interleukin 10 (IL-10) polypeptide, wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group, and wherein said IL-10 homologue has the amino acid sequence as set forth in SEQ ID NO:10, or the amino acid sequence as set forth in SEQ ID NO:10 including one or more conservative amino acid substitutions, or the ligand that selectively binds to said isolated homologue of IL-10.
(canceled)
An isolated nucleic acid, wherein the nucleic acid sequence corresponds to any one of SEQ ID Nos:2 to 9.
A vaccine, wherein said vaccine comprises a purified nucleic acid sequence encoding a homologue of human interleukin 10 (IL-10), wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group, and wherein said nucleic acid sequence is as set forth in SEQ ID NO:1, or an isolated homologue of human interleukin 10 (IL-10) polypeptide, wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group, and wherein said IL-10 homologue has the amino acid sequence as set forth in SEQ ID NO:10, or the amino acid sequence as set forth in SEQ ID NO:10 including one or more conservative amino acid substitutions, or a ligand that selectively binds to said isolated homologue of IL-10 , together with a pharmaceutically acceptable carrier, adjuvant and/or diluent.
A method of cleansing a biological sample of infection by a virus of the herpesvirideae group, the method comprising:
- (a) contacting said biological sample with an isolated ligand that selectively binds to an isolated homologue of human interleukin 10 (IL-10) polypeptide, wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group, and wherein said IL-10 homologue has the amino acid sequence as set forth in SEQ ID NO:10. or the amino acid sequence as set forth in SEQ ID NO:10 including one or more conservative amino acid substitutions,
- (b) detecting the presence of the ligand bound to a cell expressing an isolated homologue of human interleukin 10 (IL-10) polypelptide, wherein said IL-10 homologue is expressed during the latent phase of infection by a virus of the herpesvirideae group, and wherein said IL-10 homologue has the amino acid sequence as set forth in SEQ ID NO:10, or the amino acid sequence as set forth in SEQ ID NO:10 including one or more conservative amino acid substitutions, and
- (c) removing said cell to which said ligand binds.
Dependencies are inferred from the claim text.