Methods and compositions for the preparation and use of toxin conjugates
AssigneesInstitut Curie, Centre National de la Recherche Scientifique (CNRS)
InventorsLudger JOHANNES, Baldissera GIOVANI, Michel AZOULAY, Jean-Claude FLORENT
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods for the preparation of toxin conjugates that are useful in vaccination and other therapies and diagnostics. In particular, methods are provided that involve a [3+2] cycloaddition between a first reactive unsaturated group on a toxin moiety and a second reactive unsaturated group on a bioactive moiety. Also provided are conjugates that are formed through this conjugation method, pharmaceutical compositions comprising these conjugates, and methods of using these pharmaceutical compositions for the treatment or diagnostic of antigen-related conditions, including tumors and infections.
Claims33
1-42. (canceled)
A toxin conjugate comprising at least one toxin moiety covalently bound to at least one bioactive moiety, wherein covalent binding between the toxin moiety and bioactive moiety results from a [3+2] cycloaddition.
Method of preparing a toxin conjugate, the method comprising steps of:
- providing a toxin moiety comprising a first reactive unsaturated group;
- and
- contacting the toxin moiety with a bioactive moiety comprising a second reactive unsaturated group, such that a [3+2] cycloaddition occurs between the first and second unsaturated groups.
Dependencies are inferred from the claim text.