Tumor therapy with replication competent sindbis viral vectors
AssigneeNew York University
InventorsDaniel Meruelo, Jen-Chieh Tseng
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Abstract
Disclosed herein are methods for treating a mammal harboring a solid tumor which expresses higher levels of High Affinity Laminin Receptors (LAMR) than normal cells of the same lineage comprising systematically administering to a mammal in need of such treatment a therapeutically effective amount of a Replication Competent (RC) Sindbis virus vector, wherein said vector encodes a suicide gene.
Claims39
A method for treating a mammal harboring a solid tumor which expresses higher levels of High Affinity Laminin Receptors (LAMR) than normal cells of the same lineage comprising systematically administering to a mammal in need of such treatment a therapeutically effective amount of a Replication Competent (RC) Sindbis virus vector, wherein said vector encodes a suicide gene.
A method for monitoring anti-cancer therapy in a mammal harboring a solid tumor which expresses higher levels of LAMR than normal cells of the same lineage comprising administering to a mammal in need of such treatment a diagnostically effective amount of a Replication Competent (RC) Sindbis virus vector comprising a gene encoding a detectable label, and determining the amount of cancer cells in the body of said mammal, wherein the amount of cancer cells is proportional to the amount of label produced by said cancer cells and said vector encodes a suicide gene.
A method for identifying cancer cells which expresses higher levels of LAMR than normal cells of the same lineage in the body of a mammal comprising administering to a mammal in need of such treatment a diagnostically effective amount of a mut-4 RC Sindbis virus vector comprising SEQ. ID NOS.:
- 2-8 and further comprising a gene encoding a detectable label, and assaying for said label, wherein said cell is a cancer cell if it expresses said label and said vector encodes a suicide gene.
A method for determining the amount of cancer cells which express higher levels of LAMR than normal cells of the same lineage in the body of a mammal comprising the steps of (a) administering to a mammal in need of such treatment a diagnostically effective amount of a mut-4 RC Sindbis virus vector comprising SEQ. ID NOS.:
- 2-8 and further comprising a gene encoding a detectable label, and (b) determining the amount of said detectable label, wherein the amount of cancer cells in the body of said mammal is proportional to the amount of said label and the vector encodes a suicide gene.
A Replication competent mut-4 Sindbis virus vector comprising SEQ. ID NOS.:
- 2-8.
A pharmaceutical formulation or dosage form for administration to a mammal suffering from a solid tumor which expresses higher levels of LAMR than normal cells of the same lineage comprising a mut-4 RC Sindbis virus vector comprising SEQ. ID NOS.:
- 2-8 and a pharmaceutically acceptable carrier or diluent, wherein said vector further comprises a suicide gene.
Dependencies are inferred from the claim text.