Pixel classification in image analysis

Fluorescence microscopy device, comprising:

• a fluorescence microscope, providing an image of a sample;
• intensity measurement means arranged to determine a digital value of discretized intensity measures, within at least two predetermined wavelength intervals, of light coming from an imaged position;
• and
• an image analyser connected to said intensity measurement means, said image analyser comprising:
• means for obtaining a plurality of pixel vectors from said intensity measurement means,
• each said pixel vector having n intensity elements associated with a same respective imaged position, where n≧2;
• said n intensity elements representing said determined digital values;
• means for creating an angle histogram, in n−1 dimensions, of angles, in a space spanned by unity vectors of said n intensity elements, of said pixel vectors, said angles being defined relative to said unity vectors;
• means for defining at least one angle interval corresponding to a respective pixel class, based on said angle histogram in n−1 dimensions;
• means for classifying pixel vectors in a corresponding said pixel class based on said at least one angle interval;
• and
• means for outputting said classifying of said pixel vectors.