US 2009/0212776 A1 · NUCLEAR MAGNETIC RESONANCE MEASUREMENT APPARATUS AND MEASURING METHOD USING THE SAME

Abstract

An NMR measurement apparatus adopts a circulation flow scheme using a sample solution containing a high molecular compound representing a measuring object and a low molecular compound solution containing a low molecular compound representing a ligand. The measurement apparatus comprises a mixing filter 32 for mixing the sample solution and the low molecular compound solution, a separation filter 34 for performing separation therebetween, a flow channel 1 through which the sample solution drained out of the separation filter 34 is injected to the mixing filter 32, a flow channel 2 through which the low molecular compound solution drained out of the separation filter 34 is injected to the mixing filter 32, and a flow channel 3 through which the mixture solution drained out of the mixing filter 32 is injected to the separation filter 34 by way of a reservoir 10.

Claims20

Dependent claims

A nuclear magnetic resonance measurement apparatus comprising:

a reservoir for holding a mixture solution containing a first compound and a second compound of lower molecular weight than that of the first compound;
a magnet for applying a magnetic field to said mixture solution held in said reservoir;
a nuclear magnetic resonance probe attached to said reservoir;
a mixing filter for mixing a first solution containing said first compound and a second solution containing said second compound and draining a resultant solution as said mixture solution;
a separation filter for performing separation between said first solution and said second solution from said mixture solution injected thereto;
and
pipes for constituting a first flow channel through which said first solution drained out of said separation filter is injected to said mixing filter, a second flow channel through which said second solution drained out of said separation filter is injected to said mixing filter and a third flow channel through which said mixture solution drained out of said mixing filter is injected to said separation filter by way of said reservoir.

4 more elements

A measuring method using a nuclear magnetic resonance measurement apparatus having:

a reservoir for holding a mixture solution containing a first compound and a second compound of lower molecular weight than that of the first compound;
a magnet for applying a magnetic field to said mixture solution held in said reservoir;
a nuclear magnetic resonance probe attached to said reservoir;
a mixing filter for mixing a first solution containing said first compound and a second solution containing said second compound;
a separation filter for performing separation between said first solution and said second solution;
a first flow channel through which said first solution drained out of said separation filter is injected to said mixing filter;
a second flow channel through which said second solution drained out of said separation filter is injected to said mixing filter;
and
a third flow channel through which said mixture solution drained out of said mixing filter is injected to said separation filter by way of said reservoir,
wherein a first concentration meter is provided for said third flow channel laid between an injection port of said reservoir and a drainage port of said mixing filter, a second concentration meter is provided for said third flow channel laid between a drainage port of said reservoir and an injection port of said separation filter, and a third concentration meter is provided for said second flow channel,
said measuring method comprising the steps of:
(a) circulating a buffer liquid through said first to third flow channels;
(b) starting, after the step (a), measurements by said first to third concentration meters;
(c) injecting, after the step (b), said first solution to said first flow channel, circulating said first solution through said first and third flow channels and causing said first and second concentration meters to measure a uniform concentration state of said first solution;
(d) injecting, after the step (c), said second solution to said second flow channel, circulating said second solution through said second and third flow channels and causing said third concentration meter to measure a uniform concentration state of said second solution;
(e) measuring, after the step (d), nuclear magnetic resonance of said mixture solution by means of said nuclear magnetic resonance probe;
and
(f) changing, after the step (e), the concentration of said second solution and performing said steps (d) and (e) repetitively.

15 more elements

A measuring method using a nuclear magnetic resonance measurement apparatus having:

a reservoir for holding a mixture solution containing a first compound and a second compound of lower molecular weight than that of the first compound;
a magnet for applying a magnetic field to said mixture solution held in said reservoir;
a nuclear magnetic resonance probe attached to said reservoir;
a mixing filter for mixing a first solution containing said first compound and a second solution containing said second compound;
a separation filter for performing separation between said first solution and said second solution;
a first flow channel through which said first solution drained out of said separation filter is injected to said mixing filter;
a second flow channel through which said second solution drained out of said separation filter is injected to said mixing filter;
and
a third flow channel through which said mixture solution drained out of said mixing filter is injected to said separation filter by way of said reservoir,
wherein a first concentration meter is provided for said third flow channel laid between an injection port of said reservoir and a drainage port of said mixing filter, a second concentration meter is provided for said third flow channel laid between a drainage port of said reservoir and an injection port of said separation filter, and a third concentration meter is provided for said second flow channel,
said measuring method comprising the steps of:
(a) circulating a buffer liquid through said first to third flow channels;
(b) starting, after the step (a), measurements by said first to third concentration meters;
(c) injecting, after the step (b), said second solution to said second flow channel, circulating said second solution through said second and third flow channels and causing said third concentration meter to measure a uniform concentration state of said second solution;
(d) injecting, after the step (c), said first solution to said first flow channel, circulating said first solution through said first and third flow channels and causing said first and second concentration meters to measure a uniform concentration state of said mixture solution;
(e) measuring, after the step (d), nuclear magnetic resonance of said mixture solution by means of said nuclear magnetic resonance probe;
and
(f) changing, after the step (e), the concentration of said second solution and performing said steps (d) and (e) repetitively.

15 more elements

Dependencies are inferred from the claim text.

Nuclear magnetic resonance measurement apparatus and measuring method using the same

Life of this patent

Nuclear magnetic resonance measurement apparatus and measuring method using the same

Life of this patent

Abstract

Claims20