Method for producing biodiesel using high-cell-density cultivation of microalga Chlorella protothecoides in bioreactor
OwnerQingyu Wu, Wenguang Zhou +1inventors
InventorsQingyu Wu, Wenguang Zhou, Wei Xiong
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Abstract
A method is provided to produce biodiesel from algae using a strain of microalga chlorella protothecoids, by screening a specific strain with characteristics of high yield of biomass and high oil content, cultivating the screened strain for high-cell-density growth for up to 108 grams of dry cell weight per liter of the suspension in a bioreactor using solutions containing carbohydrates as feed, harvesting and drying the high density cultivated algal cells to extract oil from the dried algal cells, and producing the biodiesel by reaction of catalyzed transesterification using the extracted oil as feedstock.
Claims20
A method for producing biodiesel from algae using microalga chlorella Protothecoides in bioreactors, comprising the steps of:
- screening a specific strain of said chlorella Protothecoides with characteristics of high yield of biomass and high oil content;
- inoculating the strain in a first bioreactor for algal-seed-cells cultivating;
- feeding a first solution containing carbohydrates into the first bioreactor;
- transferring the cultivated algal-seed-cells into a second bioreactor for high-cell-density cultivation;
- feeding a second solution containing carbohydrates into the second bioreactor;
- harvesting the high density cultivated algal cells from the second bioreactor;
- drying the high density cultivated algal cells;
- extracting oil from dried algal cells;
- and
- producing the biodiesel by reaction of transesterification using the extracted oil as feedstock.
Dependencies are inferred from the claim text.