US 2009/0175906 A1 · Genetically Modified Attenuated Vesicular Stomatitis Virus, Compositions and Methods of use Thereof

Abstract

The present invention relates to methods for generating genetically modified and attenuated strains of vesicular stomatitis virus (VSV) for use in the preparation of immunogenic compositions. More particularly, the invention relates to the identification of particular genetic modifications of attenuated VSV that result in an increased yield of virus and an increase in stability of the attenuated strains for preparation of the immunogenic compositions. Methods for cell culture propagation and use in large scale production of VSV is also disclosed.

Claims50

Dependent claims

An isolated, genetically modified vesicular stomatitis virus (VSV) having at least one amino acid mutation in a region corresponding to at least one of the following positions:

the amino acids at positions 119 or 142 of the M protein;
the amino acids at positions 109, 224, 438, 477, or 481 of the G protein;
and
the amino acids at positions 205, 220 or 1450 of the L protein.

1 more element

Dependencies are inferred from the claim text.

Genetically Modified Attenuated Vesicular Stomatitis Virus, Compositions and Methods of use Thereof

Life of this patent

Genetically Modified Attenuated Vesicular Stomatitis Virus, Compositions and Methods of use Thereof

Life of this patent

Abstract

Claims50