Metabolic engineering of lipid metabolism by improving fatty acid binding and transport
AssigneeThe Salk Institute for Biological Studies
InventorsFlorence Pojer, Joseph P. Noel, Elise Larsen, Marianne Bowman, Stephane Richard
Life of this patent
Estimating the term…
Abstract
CHI like fatty acid binding proteins and genes, recombinant cells and organisms, methods of metabolic pathway engineering to improve lipid production in cells, Crystal structures of CHI like fatty acid binding proteins, methods of engineering CHI like fatty acid binding proteins and systems thereof are provided.
Claims43
A recombinant cell that expresses a heterologous chalcone isomerase like fatty acid binding protein gene, which gene encodes a chalcone isomerase like fatty acid binding protein that binds to a fatty acid in the cell.
A recombinant cell that expresses a heterologous regulator of a chalcone isomerase like fatty acid binding protein gene.
An expression vector that encodes a chalcone isomerase like fatty acid binding protein.
An isolated chalcone isomerase like fatty acid binding protein.
A crystal comprising a chalcone isomerase like fatty acid binding protein.
A method of making a recombinant cell, the method comprising:
- introducing a recombinant gene into a cell, which recombinant gene encodes a recombinant chalcone isomerase like fatty acid binding protein;
- and,
- expressing the recombinant gene in the resulting recombinant cell.
A method of modulating lipid content of a cell, the method comprising:
- expressing a recombinant chalcone isomerase like fatty acid protein gene in the cell, or expressing a heterologous modulator of a chalcone isomerase like fatty acid protein gene in the cell;
- wherein expression of the recombinant gene or heterologous modulator modulates lipid content of the cell.
A method of selecting a plant for lipid content, the method comprising:
- identifying a polymorphism in a plant population that correlates with a phenotype encoded by a chalcone isomerase like fatty acid binding protein gene;
- and,
- performing marker assisted selection of the population to select a plant in the population for the polymorphism.
A method of modifying a chalcone isomerase like fatty acid binding protein, the method comprising:
- accessing an information set derived from a crystal structure of the protein or homolog thereof, and, based on information in the information set, predicting whether making a change to the structure of the protein will increase or decrease binding to a fatty acid binding protein ligand;
- and,
- modifying the protein based upon on said predicting.
A system comprising an information storage module comprising an information set derived from a crystal structure of a chalcone isomerase like fatty acid binding protein.
Dependencies are inferred from the claim text.