Enhanced zein reduction in transgenic corn seed
AssigneeMonsanto Technology LLC
InventorsMichael H. Luethy, Thomas M. Malvar, Shihshieh Huang
Life of this patent
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Abstract
Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
Claims18
1-4. (canceled)
A recombinant DNA construct for suppression of more than one maize zein target gene that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element from more than one maize zein target gene, the anti-sense-oriented DNA elements designed in 5′ to 3′ order element 1, element 2, and at least one sense-oriented DNA element from at least one maize zein target gene, designated element 3, wherein the anti-sense-oriented DNA element 2 has at least a portion of its sequence at its 3′ end that is not complementary either to itself or to the sense-oriented DNA element 3, and sense-oriented RNA transcribed by the sense-oriented DNA element is complementary to the 5′-most end of anti-sense-oriented RNA transcribed by the anti-sense-oriented DNA element(s).
Dependencies are inferred from the claim text.