Compositions and methods for modulating lysine production
OwnerCharles Gilvarg, Thomas Leustek +1inventors
InventorsCharles Gilvarg, Thomas Leustek, Andre Hudson
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Abstract
Compositions and methods which modulate LL-diaminopimelate aminotransferase are disclosed. Also provided are compositions and methods for enhancing lysine biosynthesis in a cell.
Claims34
A nucleic acid molecule encoding an LL-DAP amino transferase.
The method of claim 25, wherein said amino transferase encoding nucleic acid further comprises sequences suitable for expression and production of said amino transferase in the plant plastid.
A method for identifying a compound which modulates LL-diaminopimelate aminotransferase activity comprising:
- a) incubating said aminotransferase in the presence and absence of said compound under conditions which promote aminotransferase activity;
- b) determining the amount of product formed in the presence and absence of said test compound, compounds which alter the amount of product formed having LL-diaminopimelate aminotransferase modulating activity.
A method of enhancing the conversion of tetrahydrodipicolinate to L,L-diaminopimelate in a cell comprising:
- a) introducing a nucleic acid encoding DAP dehydrogenase into a plant cell, or
- b) introducing a plurality of nucleic acids encoding DapC, Dap D and Dap E into said cell.
(canceled)
Dependencies are inferred from the claim text.