Compositions and methods for activating genes of interest
OwnerCharles Allen Black, JR.inventor
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Abstract
Compositions and methods for activating genes of interest are provided. The compositions comprise a masked targeted expression cassette which expresses a gene product only in the presence of a target molecule. The cassettes are useful for the treatment of disease and for preventing the proliferation of neoplastic cells.
Claims12
A masked expression cassette comprising a double stranded nucleic acid molecule wherein a first strand comprises an RNA sequence which codes for a protein of interest linked downstream of a flanking sequence, and a translation initiation site operably inserted upstream of the RNA sequence;
- and,
- the second strand bound to the flanking sequence, wherein said second strand comprises a polynucleotide corresponding to said first strand and to a target molecule.
A masked expression cassette comprising a double stranded nucleic acid molecule comprising a first strand comprising a 5′ sense sequence and a 3′ sense sequence;
- said first strand further comprising an RNA sequence which codes for a protein of interest linked downstream of said 5′ sense sequence, and a translation initiation site operably inserted upstream of the RNA sequence;
- and,
- a second strand bound to said 5′ sense sequence and to said 3′ sense sequence, wherein said second strand comprises a polynucleotide corresponding to said 5′ sense sequence and to said 3′ sense sequence and to a target molecule.
A masked expression cassette comprising a double stranded nucleic acid molecule wherein a first strand comprises an RNA sequence which codes for a protein of interest linked downstream of a plurality of sense sequences, and a translation initiation site operably inserted upstream of the RNA sequence;
- and,
- a plurality of second strands, each bound to an individual sense sequence, wherein each of said second strands comprises a nucleotide sequence corresponding to one of said sense sequences and to a target molecule.
Dependencies are inferred from the claim text.