Circular Chromosomes
OwnerLarry Gilbertson, Jason Wardinventors
Life of this patent
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Abstract
Circular chromosomes with centromere DNA comprise recombination sites flanking a region with bacterial replication DNA and a recombinase transcription unit. Circular chromosomes without bacterial replication DNA are formed by removing bacterial replication DNA from a circular chromosome by action of a recombinase that excises DNA between said recombination sites.
Claims17
A method of removing DNA from a circular chromosome in a plant cell wherein said circular chromosome comprises a plant centromere DNA region and further comprises a bacterial replication DNA region that is flanked by recombination sites, said method comprising providing in said plant cell a recombinase transcription unit comprising a promoter that is functional in said plant cell and is operably linked to DNA encoding a recombinase that will excise DNA between said recombination sites whereby, when said recombinase transcription unit is expressed in said plant cell to produce said recombinase, said bacterial replication DNA region is removed from said circular chromosome.
A method of claim 2, wherein said recombinase transcription unit is located on said circular chromosome.
A circular chromosome comprising a centromere DNA region and a bacterial replication DNA region flanked by recombination sites and wherein said circular chromosome further comprises a recombinase transcription unit comprising a promoter that is functional in said plant cell and is operably linked to DNA encoding a recombinase that will excise DNA flanked by said recombination sites.
A circular chromosome comprising a centromere DNA region and a bacterial replication DNA region flanked by meganuclease sites, wherein said circular chromosome further comprises a meganuclease transcription unit comprising a promoter active in plant cells operably linked to DNA for expressing a meganuclease whereby, when said circular chromosome is inserted into a plant cell, the meganuclease will be produced and effect excision of the DNA between said meganuclease recognition sites to linearize said circular chromosome.
Dependencies are inferred from the claim text.