AIMP2-DX2 Gene and SiRNA Targeting AIMP2-DX2
AssigneeSunghoon Kim
InventorsSunghoon Kim, Jin Woo Choi
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Abstract
The present invention relates to a variant of AIMP2 lacking exon 2 gene, named as AIMP2-DX2 gene, which is specifically expressed in cancer cells. The AIMP2-DX2 gene and siRNA targeting AIMP2-DX2 can be successfully used in the development of diagnosis and treatment of cancer.
Claims28
An isolated siRNA (small interfering RNA) molecule comprising a sense region and an antisense region that down regulates expression of an AIMP2-DX2 gene via RNA interference (RNAi), wherein each strand of the siRNA molecules is independently about 18 to about 28 nucleotides in length, wherein one strand of the siRNA molecule comprises nucleotide sequence having sufficient complementarity to an RNA of the AIMP2-DX2 gene for the siRNA molecule to direct cleavage of the RNA via RNA interference.
A recombinant nucleic acid construct comprising a nucleic acid that is capable of directing transcription of a small interfering RNA (siRNA), the nucleic acid comprising:
- (a) at least one promoter;
- (b) a DNA polynucleotide segment that is operably linked to the promoter, the segment comprising nucleotide sequence having sufficient complementarity to an RNA of the AIMP2-DX2 gene for the siRNA molecule to direct cleavage of the RNA via RNA interference;
- (c) a linker sequence comprising at least 4 nucleotides operably linked to the DNA polynucleotide segment of (b);
- and (d) operably linked to the linker sequence a second polynucleotide of at least 18 nucleotides that is complementary to the segment of (b).
An isolated nucleic acid molecule encoding the AIMP2-DX2 protein having an amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 17.
An antibody specific to the AIMP2-DX2 protein having an amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 17.
Dependencies are inferred from the claim text.