US 2009/0156533 A1 · RNA INTERFERENCE MEDIATED INHIBITION OF STROMAL CELL-DERIVED FACTOR-1 (SDF-1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)

Abstract

The present invention relates to compounds, compositions, and methods for the study, diagnosis, and treatment of traits, diseases and conditions that respond to the modulation of stromal cell-derived factor-1 (SDF-1) gene expression and/or activity. The present invention is also directed to compounds, compositions, and methods relating to traits, diseases and conditions that respond to the modulation of expression and/or activity of genes involved in SDF-1 gene expression pathways or other cellular processes that mediate the maintenance or development of such traits, diseases and conditions. Specifically, the invention relates to small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating or that mediate RNA interference (RNAi) against SDF-1 gene expression. Such small nucleic acid molecules are useful, for example, in providing compositions for treatment of traits, diseases and conditions that can respond to modulation of SDF-1 expression in a subject, such as ocular disease, cancer and proliferative diseases and any other disease, condition, trait or indication that can respond to the level of SDF-1 in a cell or tissue.

Claims20

Dependent claims

A chemically modified nucleic acid molecule, wherein:

(a) the nucleic acid molecule comprises a sense strand and a separate antisense strand, each strand having one or more pyrimidine nucleotides and one or more purine nucleotides;
(b) each strand of the nucleic acid molecule is independently 18 to 27 nucleotides in length;
(c) an 18 to 27 nucleotide sequence of the antisense strand is complementary to a human stromal cell-derived factor-1 (SDF-1) RNA sequence comprising SEQ ID NO:
907;
(d) an 18 to 27 nucleotide sequence of the sense strand is complementary to the antisense strand and comprises an 18 to 27 nucleotide sequence of the human SDF-1 RNA sequence;
and
(e) 50 percent or more of the nucleotides in each strand comprise a 2′-sugar modification, wherein the 2′-sugar modification of any of the pyrimidine nucleotides differs from the 2′-sugar modification of any of the purine nucleotides.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

12/169,519Filed 8 Jul 2008

Priority

20 Feb 2002

Published

18 Jun 2009

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C07H 21/02
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of…: with…
C12N 15/1137
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming…: against enzymes
A61K 48/00
Medicinal preparations containing genetic material which is inserted into cells of the
C12N 2310/14
Type of nucleic acid: interfering nucleic acids [NA]

Family

Application60/358,580Us provisional application · filed 20 Feb 2002

Application60/363,124Us provisional application · filed 11 Mar 2002

Application60/386,782Us provisional application · filed 6 Jun 2002

Application60/406,784Us provisional application · filed 29 Aug 2002

Application60/408,378Us provisional application · filed 5 Sep 2002

Application60/409,293Us provisional application · filed 9 Sep 2002

Application60/440,129Us provisional application · filed 15 Jan 2003

Application10/444,853Continuation in part · filed 23 May 2003

Application10/693,059Continuation in part · filed 23 Oct 2003

Application10/720,448Continuation in part · filed 24 Nov 2003

Application10/757,803Continuation in part · filed 14 Jan 2004

Application60/543,480Us provisional application · filed 10 Feb 2004

Application10/826,966Continuation in part · filed 16 Apr 2004

Application10/923,536Continuation in part · filed 20 Aug 2004

Application11/140,328Continuation · filed 27 May 2005

Application12/169,519Continuation

Application11/140,328Continuation in part

US 2004/0192626 A1Published application

US 2005/0020525 A1Published application

US 2005/0032733 A1Published application

US 2006/0019917 A1Published application

US 2007/0042983 A1Published application

US 2008/0039414 A1Published application

US 2009/0137500 A1Published application

US 8,202,979 B2Related patent

US 8,273,866 B2Related patent

US 2009/0156533 A1This patent

From the US filing history. Foreign family members aren’t listed.

RNA INTERFERENCE MEDIATED INHIBITION OF STROMAL CELL-DERIVED FACTOR-1 (SDF-1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)

Life of this patent

RNA INTERFERENCE MEDIATED INHIBITION OF STROMAL CELL-DERIVED FACTOR-1 (SDF-1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)

Life of this patent

Abstract

Claims20