Dimeric or Multimeric Microproteins
AssigneeNascaCell Technologies AG
InventorsHarald Kolmar, Ernst Boehnlein, Alexander Wentzel, Hans-Ulrich Schmoldt
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Abstract
Disclosed is a polypeptide comprising at least two microproteins, which preferably comprise an amino acid sequence having a specific binding activity to a target protein. Furthermore, disclosed are polynucleotides encoding such a polypeptide as well as pharmaceutical compositions and kits comprising said polypeptide or polynucleotide. Also disclosed herein are methods of treatments and second medical uses applying the disclosed polypeptide or polynucleotide. Additionally, the disclosure of the present application relates to a method for forming a covalent bond in a microprotein which can be used for producing the disclosed polypeptides.
Claims44
A polypeptide comprising at least two microproteins.
(canceled)
A method for forming a covalent bond in a microprotein comprising:
- (a) providing a microprotein substrate comprising an N-terminal reactive carbonyl group and a C-terminal homoserine lactone residue;
- and
- (b) reacting the microprotein substrate so as to convert said N-terminal group and said C-terminal residue into a hydrazone linkage.
The method of claim 43, wherein the microprotein substrate comprises at least two microproteins and the covalent bond formation is for generating a macrocyclic dimeric or oligomeric microprotein.
Dependencies are inferred from the claim text.