Assay for the detection of factors that modulate the expression of inagp
AssigneeGMP Endotherapeutics, Inc.
InventorsDavid A. Taylor-Fishwick, Aaron I. Vinik
Life of this patent
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Abstract
A reporter construct contains mammalian INGAP 5′-regulatory region or a fragment thereof, a minimal promoter element from mammalian INGAP or a heterologous promoter, and a reporter gene. The reporter construct can be used to screen for agents which alone or in combination up-regulate or down-regulate reporter gene expression. Alternatively, the reporter construct can be used to screen for agents that bind to the hamster INGAP 5′-regulatory region or a fragment thereof.
Claims16
A method for identifying agents which modulate INGAP expression, comprising:
- contacting a host cell comprising a reporter construct having at least one INGAP regulatory region and a region encoding a detectable product with a test agent;
- determining expression of the detectable product in the cell;
- and identifying the test agent as a modulator of INGAP expression if the test agent modulates expression of the detectable product in the cell.
The method of claim 6 wherein the regulatory sequence comprises nucleotide sequences 1-3137 of SEQ ID NO:
- 2
An in vitro method for identifying agents which modulate INGAP expression, comprising:
- a. contacting a reporter construct having at least one INGAP regulatory region and a region encoding a detectable product with a test substance under conditions sufficient for transcription and translation of said nucleotide sequence;
- determining expression of the detectable protein or nucleic acid product;
- and identifying the test substance as a modulator of INGAP expression if the test substance modulates expression of the detectable product.
An in vitro method for identifying agents which modulate INGAP expression, comprising:
- contacting the SEQ ID NOS 1, 2, 23, 32, 35, 37, 28, 24, 25, 26, 27, 29, 30, 31, 33, 34, 38, and 36 or fragments thereof with a test agent;
- determining binding of the test agent to the nucleic acid;
- and identifying the test agent as a potential modulator of INGAP expression if the test agent binds to the nucleic acid.
A method for inducing INGAP expression in a mammal in need thereof, comprising administering to the mammal an effective amount of a factor that stimulates INGAP expression in the said mammal.
Dependencies are inferred from the claim text.