US 2009/0156428 A1Published application

Multi-mode microarray apparatus and method for concurrent and sequential biological assays

OwnerAlastair J. Malcolminventor

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Abstract

A multi-mode, multiplexed array of probe elements of various forms attached to a common support and methods of using the array are disclosed. The array is used for the concurrent and/or sequential detection of combinations of more than one chemical and/or biological material, such as: target nucleic acid sequences, genomic DNA, pcr products, RNA (including microRNA), single nucleotide polymorphisms, proteins, peptides, carbohydrates, polysaccharides, phosphorylation or methylation state of target molecules, chromosomal abnormalities, and other biomolecules, moieties, metals, or chemical compounds in a biological sample. The method of the invention includes the deposition and/or in-situ synthesis of the various probe elements which comprise the array or group of arrays, the preparation and delivery of the target biological sample in forms suitable for concurrent and/or sequential detection, the hybridization methods of target to probe, the detection methods for target-probe interactions, and the use of such arrays in automated systems for research or diagnostic applications.

A method for detection of multiple targets of different types using a multi-mode array on a common support, the method comprising the steps of:

(a) providing an array with probes on a support, the array comprising a combination of two or more of the following types, on or within a common support;
and
(i) DNA (all forms, single or double strand, natural or synthetic),
(ii) RNA (all forms, single or double strand, natural or synthetic),
(iii) oligonucleotides,
(iv) PCR (“polymerase chain reaction”) amplicons,
(v) LNA (locked nucleic acid),
(vi) PNA (peptide nucleic acid),
(vii) TNA (threose nucleic acid),
(viii) PMO (phosphorodiamidate morpholino oligo),
(ix) proteins (natural or synthetic),
(x) peptides (natural or synthetic),
(xi) carbohydrates,
(xii) polysaccharides,
(xiii) cells,
(xiv) tissues,
(xv) antibodies,
(xvi) antigens,
(xvii) protein-DNA complexes,
(xviii) protein-RNA complexes,
(xix) protein-protein complexes,
(xx) DNA-RNA complexes,
(xxi) aptamers,
(xxii) dyes and dye complexes,
(xxiii) stains,
(xxiv) enzymes,
(xxv) ubiquitin, and ubiquitinylated proteins,
(xxvi) reagents to promote probe-target reactions
(b) providing the detection capability for target molecules and phenomena of the following types, individually or in combination:
(i) DNA (all forms, single or double strand, natural or synthetic),
(ii) RNA (all forms, single or double strand, natural or synthetic),
(iii) oligonucleotides,
(iv) PCR amplicons,
(v) proteins (natural or synthetic),
(vi) peptides (natural or synthetic),
(vii) carbohydrates,
(viii) polysaccharides,
(ix) cells,
(x) tissues,
(xi) antibodies,
(xii) antigens,
(xiii) metals,
(xiv) moieties,
(xv) methylated DNA,
(xvi) CpG islands,
(xvii) protein-DNA complexes,
(xviii) protein-RNA complexes,
(xiv) protein-protein complexes,
(xx) DNA-RNA complexes,
(xxi) phosphorylated DNA,
(xxii) microRNA
(xxiii) aptamers,
(xxiv) chromosomal abnormalities,
(xxv) single nucleotide polymorphisms,
(xxvi) ubiquitin, and ubiquitinylated proteins

52 more elements

Abstract

Claims18