Loading patent…
AssigneeMassachusetts Institute of Technology
InventorsShuguang Zhang, Andreas Mershin, Liselotte Kaiser, Brian Cook, Johanna F. Graveland-Bikker, Manu Prakash, David Kong, Yael Maguire
Estimating the term…
The invention provides a bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—on a support, a real time receptor-ligand binding detection method, an odorant delivery system and an odorant recognition program. The biologically-derived G-protein coupled receptor can be stabilized on nanotechnology using surfactant peptide. The said nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.
A bio-sensing nanodevice comprising:
A microfluidic chip [11] for use in detecting a test composition comprises at least one stabilized biologically-derived G-protein coupled receptor [2] immobilized on a support, and arranged in at least two dimensional microarray system.
A method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip.
A method of fabricating hOR17-4 olfactory receptor using expression system selected from cell-free, bacteria (E. coli) and yeast and human embryonic kidney cell 293 (HEK293) and its derivatives HEK293S (suspension).
Dependencies are inferred from the claim text.