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US 2009/0156423 A1Published application
Method and device for integrated synthesis and analysis of analytes on a support
AssigneeFebit AG
InventorsCord F. STAHLER, Ramon Guimil, Matthias Scheffler, Peer F. Stahler, Anke Heidbrede
Life of this patent
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Abstract
Methods and devices provide integrated synthesis of a support for analyte determination and the analyte determination. Preferred embodiments involve particles immobilized on the surface of a support to which receptors are coupled location-specifically, time-specifically or both on pre-determined positions on the support.
Claims62
A method for integrated synthesis and analyte determination on a support, comprising:
- a) providing a support body (2),
- b) conducting a liquid containing particles (46) into or onto the support body (2),
- c) immobilizing the particles (46) on at least one inner or outer surface (45) of the support body (2),
- d) conducting a liquid which contains receptors or building blocks (A, B) for synthesizing polymeric receptors over the immobilized particles (46),
- e) coupling the receptors or receptor building blocks (A, B) location-specifically, time-specifically or both to the immobilized particles (46) at predetermined positions of the support body (2),
- f) optionally repeating (d) and (e), until the desired receptors have been synthesized on the immobilized particles (46) at predetermined positions of the support body,
- g) contacting the receptors with a sample containing the analyte to be determined, and
- h) determining the analyte via binding to the receptors which are coupled to the immobilized particles (46).
5 more elements
A method for synthesizing receptors on a support, comprising:
- a) providing a support body (2),
- b) conducting a liquid containing particles (46) into or onto the support body (2),
- c) immobilizing the particles (46) on at least one inner or outer surface (45) of the support body (2),
- d) conducting a liquid which contains receptors or building blocks (A, B) for synthesizing polymeric receptors over the immobilized particles (46),
- e) coupling the receptors or receptor building blocks (A, B) location-specifically, time-specifically or both to the immobilized particles (46) at predetermined positions of the support body (2), and
- f) optionally repeating (d) and (e), until the desired receptors have been synthesized on the immobilized particles (46) at predetermined positions of the support body.
3 more elements
A method for determining one or more analytes on a support, comprising:
- a) providing a support body (2) that has at least one inner or outer surface (45), wherein particles (46) with receptors coupled thereto are immobilized at the said surface (45), and wherein said particles comprise a plurality of particle species with different receptors coated thereon,
- b) contacting said support body or said receptors with a sample containing an analyte to be determined, and
- c) determining said analyte via binding to said receptors.
A method for integrated synthesis and analyte determination on a support, comprising:
- a) providing an integrated apparatus which comprises a programmable light source matrix (4), a detector matrix (6), a support body (2) arranged between said light source matrix and said detector matrix, wherein said support body has at least one inner or outer surface (45) and has at least some transparent regions, and a means for supplying fluid into the support body and for discharging fluid from the support body,
- b) conducting a liquid containing particles (46) into or onto the support body (2),
- c) immobilizing the particles (46) on said surface (45) of the support body (2),
- d) conducting a liquid which contains receptors or building blocks (A, B) for synthesizing polymeric receptors over the immobilized particles (46),
- e) coupling the receptors or receptor building blocks (A, B) location-specifically, time-specifically or both to the immobilized particles (46) at predetermined positions of the support body (2),
- f) optionally repeating (d) and (e), until the desired receptors have been synthesized on the immobilized particles (46) at the predetermined positions of the support body,
- g) contacting the receptors with a sample containing the analyte to be determined, and
- h) determining the analyte via binding to the receptors which are coupled to the immobilized particles (46),
- wherein said synthesis of the desired receptors, said determining analytes or both are monitored and controlled in at least one position on the support.
6 more elements
A method for determining one or more analytes on a support, comprising:
- a) providing an integrated apparatus which comprises a programmable light source matrix (4), a detector matrix (6), a support body (2) which has at least one inner or outer surface (45), and which is arranged between said light source matrix and said detector matrix, wherein a plurality of particle species with different receptors are coated on said surface (45) and immobilized thereon using a magnetic or electric interaction, and a means for supplying fluid into the support body and for discharging fluid from the support body,
- b) contacting the support body or the receptors with a sample containing the analyte(s) to be determined, and
- c) determining the analyte(s) via binding to the receptors coupled to the immobilized particles (46).
A method for preparing a substance library, comprising:
- a) providing a support body (2),
- b) conducting a liquid containing particles (46) into or onto the support body (2),
- c) immobilizing said particles (46) on at least one inner or outer surface (45) of the support body (2),
- d) conducting a liquid which contains receptors or building blocks (A, B) for synthesizing polymeric receptors over the immobilized particles (46),
- e) coupling the receptors or building blocks (A, B) to the immobilized particles (46) on the support body,
- f) repeating steps (d) and (e), until the desired polymeric receptors have been synthesized on the immobilized particles (46) of the support body (2), and
- g) removing the immobilized particles (46) containing the synthesized polymeric receptors from the support body (2).
4 more elements
Dependencies are inferred from the claim text.