Library From Toxin Mutants, And Methods Of Using Same
OwnerJean Gariepy, Leigh Reversinventors
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Abstract
This application relates to libraries of ABx toxin mutants, in which a peptide insert is introduced into the protease-sensitive loop of the A-chain sequence to alter the type of cells to which toxic species are delivered. Said libraries are used in the development of therapeutics targeted against specific cell types.
Claims18
1-128. (canceled)
A combinatorial protein library comprising a plurality of protein species, each protein species comprising a modified A chain of a toxic protein into which an insert has been introduced, wherein,
- (a) the modified A chain comprises a wild-type A chain containing a mutation in at least one cysteine;
- (b) the insert is a polypeptide of varying amino acid sequence having a length of at least 2 amino acid residues;
- and
- (c) the insert is introduced into the region which would be a protease-sensitive loop in the wild-type A chain sequence.
A mutant protein comprising a modified A chain of a toxic protein into which an insert has been introduced, wherein,
- (a) the modified A chain comprises a wild-type A chain containing a mutation in at least one cysteine;
- (b) the insert is a polypeptide of varying amino acid sequence having a length of at least 2 amino acid residues;
- and
- (c) the insert is introduced into a region which would be a protease-sensitive loop in the wild type A chain sequence.
A method for isolating a toxin specific for a known target/receptor comprising the steps of:
- (a) exposing the target/receptor to a combinatorial protein library according to claim 1;
- and
- (b) isolating at least one protein species from the combinatorial protein library captured by binding to the target/receptor.
Dependencies are inferred from the claim text.