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AssigneeSiemens Healthcare Diagnostics Inc., a corporation of California
InventorsTie Q. Wei, Alan Craig, Amy Posey
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Methods and reagents are disclosed for pretreating a sample suspected of containing a hydrophobic drug for conducting an assay method for detecting the hydrophobic drug. A combination is provided in a medium. The combination comprises (i) the sample, (ii) a releasing agent for releasing the hydrophobic drug and the metabolites from endogenous binding moieties, and (iii) a selective solubility agent that provides for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The selective solubility agent comprises a water miscible, non-volatile organic solvent and is present in the medium in a concentration sufficient to provide for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The medium, which may further comprise a hemolytic agent, is incubated under conditions for releasing the hydrophobic drug and the metabolites from endogenous binding moieties. For conducting an assay for the hydrophobic drug, the above pretreatment is performed and to the medium is added reagents for determining the presence and/or amount of the hydrophobic drug in the sample wherein the reagents comprise at least one antibody for the hydrophobic drug. The medium is examined for the presence of a complex comprising the hydrophobic drug and the antibody for the hydrophobic drug, the presence and/or amount of the complex indicating the presence and/or amount of the hydrophobic drug in the sample.
A method for selectively enhancing the bioavailability of a hydrophobic drug over that of metabolites of the hydrophobic drug, the method comprising:
A method for determining an immunosuppressant drug in a sample suspected of containing an immunosuppressant drug, the method comprising:
A method for determining the presence or amount of an immunosuppressant drug in a medium suspected of containing an immunosuppressant drug, the method comprising:
Dependencies are inferred from the claim text.