High throughput drug screening method
AssigneeOptix LP
InventorsMyron J. Block, Cristobel Block
Life of this patent
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Abstract
The present invention uses the small temperature changes from reactions and utilizes them in high throughput screening methods. Briefly, a thermal block containing a series of thermally isolated wells is used so that a reaction can take place in each well without affecting the temperature of any other well. A chemical or biological reaction, such as a binding reaction, is allowed to occur in each well or chamber and the optical properties of the all of the wells are monitored using optical, preferably Kromoscopic, measurements. A determination of temperature in each of the wells from those Kromoscopic measurements can be used to determine if reaction occurred.
Claims28
A method of determining whether there is an interaction between a ligand and a target in solution, where an interaction between said ligand and said target produces a thermal change in said solution, comprising the steps of:
- allowing said ligand and said target to interact in a solution in a vessel;
- and
- optically monitoring said solution for changes in optical properties that correspond to changes in temperature, whereby a change in temperature is indicative of an interaction between said ligand and said target.
The method of claim 13 wherein a Si detector is used to monitor the optical properties of the aqueous solution in the wells.
Dependencies are inferred from the claim text.