Compositions comprising promoter sequences and methods of use
AssigneeNorth Carolina State University
InventorsRodolphe Barrangou, Andrea Azcarate-Peril, Eric Altermann, Tri Duong, Todd R. Klaenhammer
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Abstract
Nucleic acid molecules, fragments and variants thereof having promoter activity are provided in the current invention. The invention also provides vectors containing a nucleic acid molecule of the invention and cells comprising the vectors. Methods for making and using the nucleic acid molecules of the invention are further provided.
Claims21
An isolated nucleic acid molecule having promoter activity, wherein the nucleic acid molecule is selected from the group consisting of:
- (a) a nucleic acid molecule comprising a nucleotide sequence as set forth in any one of SEQ ID NOS:65 or 73;
- (b) a nucleic acid molecule that hybridizes to the complement of the nucleic acid of (a) under stringent conditions and has promoter activity, wherein said stringent conditions comprise 6×SSC, 0.1% SDS at about 45° C., followed by a wash of 0.2×SSC and 0.1% SDS at 50-65° C.;
- and
- (c) a nucleic acid molecule having at least 95% identity to the nucleotide sequence set forth in any one of SEQ ID NOS:65 or 73 and has promoter activity.
An isolated nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:6, wherein said nucleotide sequence has promoter activity.
A method to express a nucleotide sequence of interest in a cell comprising introducing into said cell a recombinant nucleic aid molecule comprising a polynucleotide having at least 95% sequence identity to SEQ ID NO:6 or comprising SEQ ID NO:6, wherein said polynucleotide has promoter activity and is operably linked to the nucleotide sequence of interest.
Dependencies are inferred from the claim text.