Method for transfer of molecular substances with prokaryontic nucleic acid-binding proteins
AssigneeACGT ProGenomics AG
InventorsGerald Boehm, Dirk Esser
Life of this patent
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Abstract
The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. Where the substance to be transferred is a nucleic acid, the protein forms a reversible complex with the nucleic acid. The prokaryotic protein condenses and compacts the nucleic acids. Said nucleic acids can be taken up in the target cells after suitable incubation.
Claims10
1-16. (canceled)
Method for transferring a nucleic acid, a nucleic acid analog, or a substance containing amino acids into prokaryotic or eukaryotic target cells, comprising the steps of:
- (1) contacting the nucleic acid or the nucleic acid analog to be transferred, or the nucleic acids or nucleic acid analogs or substances containing amino acids with a prokaryotic HU-protein or HU-protein modified by fusion with a protein, a peptide, or a protein domain, thereby forming a complex of the nucleic acid, the nucleic acid analog or the nucleic acids or nucleic acid analogs or substances containing amino acids and the prokaryotic HU-protein or the modified HU-protein, and
- (2) subsequently contacting the complex with the prokaryotic or eukaryotic target cells, thereby transferring the complex into the cells.
Dependencies are inferred from the claim text.