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AssigneeLife Technologies Corporation
InventorsMohanachari Vemuri, Sackamone Phommachanh, Katherine Wagner, Mary Tilkins
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The present invention relates to cell culture, more specifically to cell culture which may be cell culture such as stem cell culture, embryonic stem cell (ESC) culture and primary cell culture. Disclosed herein are compositions of matter, including without limitation cell matrices, matrix-forming formulations and cell cultures, wherein the cells may be cells such as stem cells, such as ESC, or primary cells, such as keratinocyte and fibroblast cells. Also disclosed herein are articles of manufacture comprising one or more of the compositions of matter of the invention, methods of making and using the compositions of matter and articles of manufacture of the invention, business methods, and tangible media comprising instructions or plans for one or more of the methods and compositions of the invention.
A composition of matter for coating a substrate with a cell matrix, wherein:
A composition of matter comprising a substrate coated with a cell matrix and a cell in adherence the cell matrix, wherein:
An article of manufacture comprising a surface coated with a stem cell matrix, wherein the stem cell matrix consists essentially of Fibronectin and Albumin at concentrations effective for forming a cellular matrix.
A stem cell culture, comprising:
An article of manufacture comprising a stem cell culture, the stem cell culture comprising:
A method of preparing an article of manufacture comprising a surface coated with a stem cell matrix, wherein the stem cell matrix consists essentially of Fibronectin and Albumin at concentrations effective for forming a cellular matrix, the method comprising the following steps, carried out simultaneously or in any order:
A method of generating a stem cell culture, comprising the following steps:
A kit comprising a carrier means compartmentalized to receive in close confinement therein one or more container means, wherein a first container means contains a matrix-forming formulation, wherein:
A kit comprising a carrier means compartmentalized to receive in close confinement therein one or more container means, wherein a first container means contains a stem cell matrix, wherein:
A kit comprising a carrier means compartmentalized to receive in close confinement therein one or more container means, wherein a first container means contains a stem cell culture, wherein the stem cell culture comprises:
Dependencies are inferred from the claim text.