Liquid-gas-phase exposure reactor for cell culturing
AssigneeProbiogen AG
InventorsUwe Marx, Marco Riedel, Hikmat Bushnaq-Josting
Life of this patent
Estimating the term…
Abstract
Initiation of growth and cultivation of cells can be performed by introducing the cells into culture compartments of a liquid-gas-phase exposure bioreactor containing a supply chamber in which there are disposed hollow-filament membranes having an inside diameter of no larger than 5 mm, wherein an inner volume of said hollow-filament membranes forms the culture compartments. Approximately one half of the supply chamber is filled with a nutrient medium and a remainder is filled with a gas mixture. Perfusion of medium and gas is turned on simultaneously or separately. The hollow-filament membranes and the cells contained therein are cyclically exposed to the gas or liquid phase.
Claims9
1-8. (canceled)
A device, comprising:
- a cylindrical two-phase supply chamber which can be charged with gas and a culture medium,
- parallel to the longitudinal axis of a shell of said supply chamber, polymeric, cell-retaining, microfiltering, hollow-filament membranes having an inside diameter of not more than 5 mm are fixed in an end plate, wherein the inner volumes of said hollow-filament membranes form culture compartments in which cells to be cultivated are disposed,
- wherein the supply chamber contains a gas phase through which a gas mixture can flow and a liquid phase through which said culture medium can flow;
- wherein each hollow-filament membrane has a spacing of at least 0.5 mm to the neighboring hollow-filament membrane over the length of the cylinder;
- wherein the hollow-filament membranes are symmetrically disposed relative to an imaginary cross section along the longitudinal axis of the cylinder;
- wherein no membrane is disposed on the imaginary cross-sectional plane along the longitudinal axis of the cylinder;
- wherein said device is capable of being used for initiation of growth and cultivation of cells.
16-17. (canceled)
Dependencies are inferred from the claim text.