Fluorescent protein
AssigneesRiken, Medical & Biological Laboratories Co., Ltd.
InventorsAtsushi Miyawaki, Ryoko Ando, Hideaki Mizuno, Satoshi Karasawa
Life of this patent
Estimating the term…
Abstract
The object of the present invention is to provide a novel fluorescent protein in which on and off of fluorescence thereof can be controlled by irradiation with lights of two different wavelengths. The present invention provides a fluorescent protein shown in the following (a) or (b); (a) a protein which has the amino acid sequence shown in SEQ ID NO: 3 or 7; or (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO: 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.
Claims18
A fluorescent protein shown in the following (a) or (b);
- (a) a protein which has the amino acid sequence shown in SEQ ID NO:
- 1 or 5;
- or
- (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO:
- 1 or 5, and which has fluorescence characteristics.
A DNA encoding the fluorescent protein shown in the following (a) or (b);
- (a) a protein which has the amino acid sequence shown in SEQ ID NO:
- 1 or 5;
- or
- (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO:
- 1 or 5, and which has fluorescence characteristics.
A DNA shown in the following (a) or (b);
- (a) DNA which has the nucleotide sequence shown in SEQ ID NO:
- 2 or 6;
- (b) DNA which has a nucleotide sequence comprising a deletion, substitution, and/or addition of one or several nucleotides with respect to the nucleotide sequence shown in SEQ ID NO:
- 2 or 6, and which has a nucleotide sequence encoding a protein that has fluorescence characteristics.
A fluorescent protein shown in the following (a) or (b);
- (a) a protein which has the amino acid sequence shown in SEQ ID NO:
- 3 or 7;
- or
- (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO:
- 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.
A DNA encoding the fluorescent protein shown in the following (a) or (b):
- (a) a protein which has the amino acid sequence shown in SEQ ID NO:
- 3 or 7;
- or
- (b) a protein which has an amino acid sequence comprising a deletion, substitution, and/or addition of one or several amino acids with respect to the amino acid sequence shown in SEQ ID NO:
- 3 or 7, and which has fluorescence characteristics of exhibiting a photochromic effect.
A DNA shown in the following (a) or (b):
- (a) DNA which has the nucleotide sequence shown in SEQ ID NO:
- 4 or 8;
- (b) DNA which has a nucleotide sequence comprising a deletion, substitution, and/or addition of one or several nucleotides with respect to the nucleotide sequence shown in SEQ ID NO:
- 4 or 8, and which has a nucleotide sequence encoding a protein that has fluorescence characteristics of exhibiting a photochromic effect.
Dependencies are inferred from the claim text.