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US 2009/0155882 A1Published application
Alpha-Amylase Variants
AssigneeNovozymes A/S
InventorsCarsten Andersen, Christel Thea Jorgensen, Henrik Bisgard-Frantzen, Allan Svendsen, Soren Kjaerulff
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Abstract
The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
Claims21
A variant of a parent Termamyl-like alpha-amylase, comprising an alteration at one or more positions selected from the group of:
- W13, G48, T49;
- S50;
- Q51, A52, D53, V54, G57, G107, G108, A111, S168, M197, wherein (a) the alteration(s) are independently
- (i) an insertion of an amino acid downstream of the amino acid which occupies the position,
- (ii) a deletion of the amino acid which occupies the position, or
- (iii) a substitution of the amino acid which occupies the position with a different amino acid,
- (b) the variant has alpha-amylase activity and (c) each position corresponds to a position of the amino acid sequence of the parent Termamyl-like alpha-amylase having the amino acid sequence of SEQ ID NO:
- 4.
5 more elements
21-25. (canceled)
Dependencies are inferred from the claim text.