US 2009/0155879 A1 · RECONSTITUTION OF 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE ACTIVITY BY FRAGMENT COMPLEMENTATION

Abstract

The present invention relates to protein fragments of 5-enolpyruvylshikimate-3-phosphate synthase, which are selected from the protein fragment pairs of EPSPS, two such protein fragments can make up full length EPSPS and reconstitute EPSPS activities by complementation without help of any joint structure. The present invention also relates to nucleic acid molecules encoding the protein fragments, expression vectors and cells comprising such nucleic acid molecules. The present invention also relates to methods for reconstituting EPSPS activities by using the fragments or the nucleic acid molecules or the expression vectors of the present invention, as well as methods for dividing the protein fragments of the present invention.

Claims9

Dependent claims

A 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein fragment selected from a pair of EPSPS protein fragments, wherein the fragment pair is comprised of two fragments which are capable, by complementation, of joining to form a structure that lacks a co-valent bond joining the fragments and that exhibits EPSPS activity.

Dependencies are inferred from the claim text.

Facts

Application

11/721,856Filed 16 Dec 2005

Priority

CN 200410102289.416 Dec 2004

Published

18 Jun 2009

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 15/8216
For plant cells , e.g. plant artificial chromosomes: methods for controlling, regulating or enhancing expression of transgenes in plant cells
C12N 9/1092
Transferring alkyl or aryl groups other than methyl groups: 3-Phosphoshikimate 1-carboxyvinyltransferase , i.e. 5-enolpyruvylshikimate-3-phosphate…
C12N 15/8257
With non-agronomic quality traits, e.g. for industrial processing; Value added, non-agronomic traits: for the production of primary gene products,…
C12N 15/8275
For herbicide resistance: glyphosate

Reconstitution of 5-enolpyruvylshikimate-3-phosphate synthase activity by fragment complementation

Life of this patent

Reconstitution of 5-enolpyruvylshikimate-3-phosphate synthase activity by fragment complementation

Life of this patent

Abstract

Claims9