Biofuel production
AssigneesBio Architecture Lab, Inc., University of Washington
InventorsYuki Kashiyama, Yasuo Yoshikuni, David Baker, Justin B. Siegel
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Abstract
Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched refinery-produced petroleum products are also provided, as well as methods for producing the same.
Claims70
A method for converting a suitable monosaccharide or oligosaccharide to a commodity chemical, comprising:
- (a) contacting the suitable monosaccharide or oligosaccharide with a commodity chemical biosynthesis pathway, wherein the commodity chemical biosynthesis pathway comprises an aldehyde or ketone biosynthesis pathway, a C—C ligation pathway, and/or a dehydration and reduction pathway,
- thereby converting the suitable monosaccharide or oligosaccharide to the commodity chemical.
A method for converting a suitable monosaccharide or oligosaccharide to a commodity chemical comprising,
- (b) contacting the suitable monosaccharide or oligosaccharide with a microbial system for a time sufficient to convert to the suitable monosaccharide or oligosaccharide to the commodity chemical, wherein the microbial system comprises;
- (i) one or more genes encoding and expressing a biosynthesis pathway;
- (ii) one or more genes encoding and expressing a C—C ligation pathway;
- and
- (iii) a reduction and dehydration pathway, comprising one or more genes encoding and expressing an enzyme selected from a diol dehydrogenase, a diol dehydratase, and a secondary alcohol dehydrogenase,
- thereby converting the suitable monosaccharide or oligosaccharide to the commodity chemical.
A recombinant microorganism, comprising (i) one or more genes encoding and expressing an aldehyde and/or ketone biosynthesis pathway;
- (ii) one or more genes encoding and expressing a C—C ligation pathway;
- and (iii) a reduction and dehydration pathway, comprising one or more genes encoding and expressing an enzyme selected from a diol dehydrogenase, a diol dehydratase, and a secondary alcohol dehydrogenase.
A recombinant microorganism, comprising one or more genes encoding and expressing an aldehyde or ketone biosynthesis pathway, wherein the pathway comprises at least one exogenous gene.
A recombinant microorganism, comprising one or more exogenous genes encoding and expressing one or more enzymes selected from a C—C ligase, a diol dehydrogenase, a diol dehydratase, and a secondary alcohol dehydrogenase.
A method for converting a suitable monosaccharide to a commodity chemical comprising,
- (a) contacting the suitable monosaccharide with a microbial system for a time sufficient to convert to the suitable monosaccharide to the commodity chemical, wherein the microbial system comprises,
- (i) one or more genes encoding and expressing a pathway selected from a fatty acid biosynthesis pathway, an amino acid biosynthetic pathway, and a short chain alcohol biosynthetic pathway;
- (ii) one or more genes encoding and expressing a keto-acid decarboxylase, aldehyde dehydrogenase, and/or alcohol dehydrogenase;
- and
- (iii) an enzymatic reduction pathway selected from (1) an enzymatic long chain alcohol reduction pathway, (2) an enzymatic decarbonylation pathway, (3) an enzymatic decarboxylation pathway, and (4) an enzymatic reduction pathway comprising (1), (2), and/or (3),
- thereby converting the suitable monosaccharide or oligosaccharide to the commodity chemical.
A recombinant microorganism, comprising (i) one or more genes encoding and expressing a pathway selected from a fatty acid biosynthesis pathway, an amino acid biosynthetic pathway, and a short chain alcohol biosynthetic pathway;
- (ii) one or more genes encoding and expressing a keto-acid decarboxylase, aldehyde dehydrogenase, and/or alcohol dehydrogenase;
- and (iii) an enzymatic reduction pathway selected from (1) an enzymatic long chain alcohol reduction pathway, (2) an enzymatic decarbonylation pathway, (3) an enzymatic decarboxylation pathway, and (4) an enzymatic reduction pathway comprising (1), (2), and/or (3).
Dependencies are inferred from the claim text.